For 24 reactions: ABL Control Gene Standards, NPM1 MutB Gene Standards, NPM1 MutD Gene Standards, Primer and Probe Mix ABL, Primer and Probe Mix NPM1 mutB Mutated Gene, Primer and Probe Mix NPM1 mutD Mutated Gene
ipsogen NPM1 mut A, B&D MutaQuant Kits为即用型试剂盒,能够定量检测人类RNA中NPM1基因转录本A型、B型和D型突变(参见NPM1 alleles)。检测结果为相对于ABL对照基因的相对定量结果(参见ABL control gene transcript)。该试剂盒灵敏度高,可重复性强。ipsogen NPM1 mut A, B&D MutaQuant Kit为ABL基因提供3个标准品稀释液,为NPM1基因提供5个标准品稀释液。
程序
第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增DNA。ipsogen NPM1 mut A, B&D MutaQuant Kits能够对NPM1基因转录本的特定突变型(A、B和D)进行可靠、灵敏的定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen NPM1 mut A, B&D MutaQuant Kits能够对NPM1基因转录本的特定突变型(A、B和D)进行可靠、灵敏的定量检测,仅适用于科研。
辅助数据和图表
Sensitive detection of NPM1 mut A and ABL standards.
Detection of [A] NPM1 mut A standards (M1 to M5) with 101, 102, 103, 105, and 106 copies/5 μl and [B] ABL standards (C1, C2, C3) with 103, 104, and 105 copies/5 μl.