QIAsymphony RGQ
用于整合的自动纯化、反应体系构建和检测，满足分子检测所需
Products
QIAsymphony SP核酸提取纯化分析仪应与可与其兼容的QIAsymphony Kits配合使用，适用于试剂盒使用手册中指定的应用。QIAsymphony AS应与可与其兼容的QIAGEN Kits配合使用，适用于试剂盒使用手册中指定的应用。如果将QIAsymphony AS 与非QIAGEN的试剂盒配合使用，用户应自行确认是否适用于相关应用。Rotor-Gene Q实时荧光定量PCR分析仪应与可与其兼容的QIAGEN Kits配合使用，适用于试剂盒使用手册中指定的应用。如果将Rotor-Gene Q实时荧光定量PCR分析仪与非QIAGEN的试剂盒配合使用，用户应自行确认是否适用于相关应用。
特点
- 前所未有的灵活性
- 优化的操作流程，高实用性
- 易于操作，提供最大程度的便利性
- 监控分析和实验室开发测试
- 适合多种应用
产品详情
新的QIAGEN工作流程包括用于样本制备的QIAsymphony SP核酸提取纯化分析仪、用于反应体系构建的QIAsymphony AS以及在Rotor-Gene Q实时荧光定量PCR分析仪上进行的一系列即用型分析。QIAGEN将进一步研发、拓展QIAsymphony系列产品。
绩效
QIAsymphony RGQ配合artus QS-RGQ Kits，应用于多种高灵敏度、高特异性检测：
- CMV DNA（参见"Highly sensitive detection of CMV DNA"）
- EBV DNA（参见"Highly sensitive detection of EBV DNA"）
- HBV DNA（参见"Highly sensitive detection of HBV DNA"）
- HIV-1 RNA（参见"Highly sensitive detection of HIV-1 RNA"）
- HCV RNA（参见"Highly sensitive detection of HCV RNA"）
原理
QIAsymphony RGQ可处理多达96个样本，从样本到结果只需一次完整的自动化运行，节省时间。一系列专门的试剂盒可组成分子检测的开放或封闭系统。
QIAsymphony SP/AS仪器和Rotor-Gene Q实时荧光定量PCR分析仪配合形成了一个可自动完成工作流程的完整系统。QIAsymphony SP核酸提取纯化分析仪可自动完成各种类型样本的制备。QIAsymphony AS确保构建的PCR反应体系高度可靠且重复性好，适合每日需要进行复杂PCR分析的实验室。QIAsymphony AS可对每次运行或样本进行多重体系构建，支持artus QS-RGQ的产品，完全兼容CE-IVD标准的工作流程，也支持自己设定的工作流程。Rotor-Gene Q实时荧光定量PCR分析仪可配合CE-IVD-marked的artus QS-RGQ试剂盒或自己设定的工作流程进行敏感、精确的real-time PCR分析。
| 试剂盒 | 仪器 | 方法 |
|---|---|---|
| QIAsymphony DSP Virus/Pathogen Kits | QIAsymphony SP核酸提取纯化分析仪 | 从多种类型的样本中纯化病毒核酸或细菌DNA |
| artus QS-RGQ Kits | QIAsymphony AS | Real-time PCR（多重；样本和内参） |
| 应用 | 试剂盒 | 仪器 | 方法 |
|---|---|---|---|
| 纯化 | QIAsymphony DSP Virus/Pathogen Kits | QIAsymphony SP核酸提取纯化分析仪 | 从各种类型样本中纯化病毒核酸或细菌 |
| QIAsymphony DSP AXpH DNA Kit | QIAsymphony SP核酸提取纯化分析仪 | 从细胞培养悬浮液中纯化病毒核酸或细菌 | |
| QIAsymphony Certal Kits | QIAsymphony SP核酸提取纯化分析仪 | 从生物纯化缓冲液、细胞培养悬浮液样本和疫苗制备样本中纯化宿主残留DNA和病毒核酸 | |
| 反应体系构建 —病原体检测 | QuantiFast Pathogen +IC Kits | QIAsymphony AS | 使用序列特异性探针进行多重real-time PCR和RT-PCR |
| QuantiTect Virus Kits | QIAsymphony AS | 可在单管中高度灵敏的检测多种病毒DNA和RNA | |
| QIAGEN OneStep RT-PCR Kit | QIAsymphony AS | 使用任何RNA模板，简单设置，进行灵敏的RT-PCR | |
| 反应体系构建 —基因表达 | Rotor-Gene SYBR® Green Kits | QIAsymphony AS | 使用SYBR® Green I进行超快速、精确的real-time PCR和RT-PCR |
| Rotor-Gene Probe Kits | QIAsymphony AS | 使用序列特异性的探针进行超快速、精确的real-time PCR和RT-PCR | |
| Rotor-Gene Multiplex Kits | QIAsymphony AS | 使用序列特异性的探针进行超快速、精确的多重real-time PCR和RT-PCR | |
| 反应体系构建 —残留DNA 检测 | Certal Detection Kits | QIAsymphony AS | 多重real-time PCR检测残留DNA和内参 |
程序
QIAsymphony RGQ工作流程的开始是在QIAsymphony SP仪器上纯化核酸。从样本制备流程中得到的含纯核酸的洗脱液转移到QIAsymphony AS模块，进行分析体系构建。分析样本再转移到Rotor-Gene Q实时荧光定量PCR分析仪进行PCR和数据分析。
为了满足不同工作流程需要，操作系统中有两种模式：完整模式或独立模式。在完整模式中，样本在QIAsymphony SP核酸提取纯化分析仪处理后直接转移到QIAsymphony AS，减少了手工操作步骤和记录。为了增加灵活性，QIAsymphony SP核酸提取纯化分析仪和AS也可在独立模式中进行相互独立的操作。参见"QIAsymphony workflows"。
应用
QIAsymphony RGQ是目前和将来用于各种分子检测的最具有多功能性的系统。QIAsymphony RGQ在一个平台上整合了多种分析，也能进行自行开发测试。
适合于开放和闭合系统多种应用，包括：
- artus移植面板（CMV、EBV、HSV、BK病毒和VZV）
- artus血源性病毒面板（HIV、HCV和HBV）
- 内部测试
Specifications
| Features | Specifications |
|---|---|
| Elution volume | QIAsymphony SP, 50–400 µl (application/protocol dependent) |
| Features | Dynamic range, 12 orders of magnitude |
| Operating temperature | 15-32°C |
| Samples per run (throughput) | Tubes 0.2 ml; Strip Tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; Up to 100 samples per run using a Rotor-Disc 100 |
| Storage temperature | 5-40°C in manufacturer's package |
| Pollution level | 2 |
| Overvoltage category | II |
| Humidity | Relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C |
| Instrument dimensions | QIAsymphony SP, 130 x 75 x 103 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm |
| Processing | Automated |
| Heat dissipation/thermal load | Average, 0.183 kW (632 BTU/hour); Peak, 0.458 kW (1578 BTU/hour) |
| Input volume | QIAsymphony SP, 100-1000 µl (application/protocol dependent) |
| Optical System | Up to 6 channels spanning UV to infra-red wavelengths; Excitation sources: High energy light-emitting diodes; Detector: Photomultiplier; Acquisition time: 4 s. The software allows to create new excitation/detection wavelength combinations |
| Software | Rotor-Gene Q software, supplied on the installation CD provided |
| Technology | QIAsymphony SP — QIAGEN magnetic-particles chemistry |
| Power | 100-240 V AC, 50-60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages |
| Place of operation | For indoor use only |
| Kits or Application Packs designed for this instrument | QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits |
| Protocols/main application on this instrument | QIAsymphony SP — Purification of DNA, RNA, bacterial and viral nucleic acids, and 6xHis-tagged protein from a wide range of starting materials; QIAsymphony AS — PCR setup. |
| Thermal performance | Temperature range, 35 to 99ºC; Temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); Temperature resolution, ±0.02ºC (smallest programmable increment); Temperature uniformity, ±0.02ºC; Ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling |
| Typical run time | 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent) |
| Weight | QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg |
| Transportation conditions | -25-70°C in manufacturer's package |
| Warranty | 1 year on instrument; lifetime guarantee on excitation LEDs |
| Altitude | Up to 2000 m (6500 ft) |
资源
安全数据表 (1)
性能数据 (10)
Application/Protocol Documents for User-Validated Use (6)
Application/Protocol Documents for IVD Use (9)
产品介绍与指南 (1)
用户使用手册 (2)
Labware Files and Documents (1)
耗材文档 (2)
应用说明 (1)
应用与实验方案 (1)
试剂盒操作手册 (2)
实验方案软件 (1)
仪器技术参数 (2)
Safety Data Sheets (1)
Certificates of Analysis (1)
Labware Documents (1)
Kit Handbooks (1)
FAQ
Is it possible to process samples on the AS-module while SP is running?