QIAseq Multimodal Pan-Cancer Panel

The only one-day, low-input sample to sequencing workflow for simultaneous and comprehensive genomic profiling of DNA variants, RNA fusions and assessing TMB/MSI in solid tumors and heme malignancies

Products

The QIAseq Multimodal Pan-Cancer Panel is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAseq Multimodal HC Panel (12)

Cat. No. / ID:  333942

QIAseq Multimodal HC Panel (12) contains ALL reagents (except indices) sufficient to process 12 samples for multimodal (DNA and RNA) sequencing; fixed high content panel for a total of 24 reactions
This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
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QIAseq Multimodal HC Panel (96)

Cat. No. / ID:  333945

QIAseq Multimodal HC Panel (96) contains ALL reagents (except indices) sufficient to process 96 samples for multimodal (DNA and RNA) sequencing; fixed high content panel for a total of 192 reactions
This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
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QIAseq Multimodal Index I SW (12)

Cat. No. / ID:  333982

QIAseq Multimodal Index I SW (12) contains DNA and RNA indexing for 12 samples using the separated workflow for targeted enrichment
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QIAseq Multimodal HT SW 96 UDI

Cat. No. / ID:  333986

QIAseq Multimodal HT SW 96 UDI contains DNA and RNA indexing for 96 samples using the separated workflow for targeted enrichment
Customize at GeneGlobeAvailable in a variety of plastics 

Features

  • QIAseq enrichment technology is the basis for Multimodal's uniform and complete coverage of targets, including complex genes like CEPBA, and confident detection of known and novel fusions
  • Pre-optimized protocol to go from total nucleic acid extraction to preparation of UMI-containing, unique dual-indexed, NGS-ready libraries using a single-day workflow to enhance sensitivity, improve error correction and reduce index hopping
  • Pre-configured automation-compatible workflow solution with secondary analysis and variant interpretation for rapid onboarding, faster turnaround time and improved scalability

Product Details

QIAseq Multimodal Panels have been developed for consolidated targeted DNA and RNA enrichment and analyses. Unlike other available approaches, QIAseq Multimodal Panels do not require 2 separate workflows for DNA and RNA analysis – saving time and conserving samples that are of limited availability.

Principle

Recent advances in NGS chemistries, platforms, and bioinformatics pipelines are enabling users to efficiently interrogate biological samples for changes in DNA and RNA. Other available approaches, however, require the use of 2 separate workflows to prepare libraries from separate DNA and RNA isolates. Limitations of such approaches include:

  • Large amounts of sample material required for generating sufficient amounts of input DNA and RNA for multiple workflows
  • Added complexity of deriving integrated insights from results of different technical approaches, each with its own innate bias
  • Inefficient use of resources
  • Long turn-around times

To overcome the limitations associated with current approaches, QIAseq Multimodal Panels start with total nucleic acids (or DNA + RNA) and prepares UDI-containing, Illumina-compatible targeted DNA and RNA libraries using a single-day, consolidated workflow. In addition, QIAseq Multimodal Panels have been designed for use with low-yield and poor-quality biological samples.

Procedure

Workflow of the QIAseq Multimodal Panels


The QIAseq Multimodal Panel workflow can be used to prepare sequencing-ready libraries in a single day. The library insert size is approximately 150 bp, making the QIAseq Multimodal Panels highly compatible with low-quality samples, such as FFPE samples.


Robust detection of DNA and RNA biomarkers


QIAseq Multimodal Panels can be used to reliably detect DNA and RNA biomarkers using a consolidated workflow from total nucleic acids. The ability of QIAseq Multimodal Panels to simultaneously detect DNA and RNA biomarkers has been benchmarked against two separate workflows (namely, QIAseq Targeted DNA Panels and QIAseq RNA Fusion XP Panels).

Applications

QIAseq Multimodal Panels can be used to interrogate different types of biomarkers using a consolidated workflow from total nucleic acids, which can be isolated using dedicated sample isolation protocols that have been developed specifically for the QIAseq Multimodal Panels.

From DNA:

  • Single nucleotide variants (SNVs)
  • Insertions and Deletions (InDels)
  • Copy number variants (CNVs)
  • TMB score
  • MSI status

 

From RNA:

  • Fusions
  • Exon skipping events
  • Gene expression levels

 

Supporting data and figures

Resources

Brochures & Guides (4)
State-of-the-art technologies to fast-track and streamline NGS workflows
QIAseq Multimodal Analytical Technology
QIAseq Multimodal: The power of one
Supplementary Files (1)
List of gene targets for QIAseq Pan-cancer Multimodal Panel
Safety Data Sheets (1)
Certificates of Analysis (1)