TissueRuptor

低通量样本破碎,适用于分子分析

Products

TissueRuptor 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

特点

  • 快速、高效的破碎各种类型的样本
  • 一次性钻头有助于消除交叉污染
  • 一次性钻头节省实验时间
  • 透明钻头确保破碎过程可监控
  • 与QIAGEN样本制备技术无缝整合

产品详情

TissueRuptor是一款手持式匀浆器,可快速、有效、灵活的对人类、动物和植物样本进行破碎,适用于多种下游应用。TissueRuptor使用透明的一次性钻头,可避免交叉污染,并且可以直观的监控样本的破碎过程。此外,使用一次性钻头可以节省每次处理样本之后的清洁时间。

绩效

QIAGEN已验证表明TissueRuptor可与QIAGEN样本制备试剂盒配合使用。使用TissueRuptor进行样本破碎确保获得最大产量的核酸和蛋白质,用于PCR、Western印迹和酶分析等下游应用(参见" Successful purification of phosphoprotein"和" High performance results in PCR")。对于多数组织,使用TissueRuptor进行破碎和匀浆达到的效果与传统转子定子式匀浆器的效果相当(参见"Pure RNA with high RIN values")。TissueRuptor可实现对组织样本的高效破碎,因此使用QIAGEN核酸纯化试剂盒可持续获得高品质DNA和RNA(参见"Pure RNA with high RIN values"、"Reproducible purification of high-quality genomic DNA"和"Efficient disruption and homogenization of animal tissues")。
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原理

基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎,以确保获得高产的DNA、RNA和蛋白质。TissueRuptor可快速、彻底的破碎样本,彻底释放生物分子,并可同时对样本进行匀质化,协助后续使用QIAGEN试剂盒进行纯化。

QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案,TissueRuptor是其中关键的组成部分。优化的实验方案整合了样本破碎和生物分子纯化,提供高效的工作流程。此外,多种自动化解决方案可用于低至高通量的生物分子纯化和分析。

QIAGEN的RNAlater RNA Stabilization Reagent可稳定RNA,Allprotect Tissue Reagent可稳定DNA、RNA和蛋白质。TissueRuptor可轻松破碎使用以上试剂采集的组织样本。

与QIAGEN破碎仪器兼容的QIAGEN纯化试剂盒

纯化的待分析物 样本类型 QIAGEN试剂盒
RNA 容易裂解的组织 RNeasy Kits
RNeasy Plus Kits
RNeasy Protect Kits
RNA 富含纤维的组织 RNeasy Fibrous Tissue Kits
RNA 各种类型组织 RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA 植物组织 RNeasy Plant Mini Kit
RNA 酵母 RNeasy Kits
RNA 细菌 RNeasy Protect Bacteria Kits
microRNA 各种类型组织 miRNeasy Kits
DNA 人类组织 QIAamp DNA Kits
DNA 动物组织 DNeasy Blood & Tissue Kits
蛋白质 组织 Qproteome Mammalian Protein Prep Kit
磷蛋白 组织 PhosphoProtein Purification Kit
糖蛋白 组织 Qproteome Glycoprotein Kits
DNA和RNA 组织 AllPrep DNA/RNA Kits
DNA、RNA和蛋白质 组织 AllPrep DNA/RNA/Protein Mini Kit

程序

TissueRuptor是一款手持式设备,带有TissueRuptor一次性钻头。钻头的刀片高速旋转,通过搅动和机械剪切作用,同时破碎和匀质化样本。适用的样本类型包括人类、动物和植物组织(对于难破碎的植物组织,建议使用TissueLyser II)。通常只需全速破碎30秒,即可释放起始样本中的核酸或蛋白质。

样本破碎完成后,可丢弃TissueRuptor的一次性钻头,使用新钻头破碎下一个样本。无需清洗、再使用同一钻头,因此可节省时间,避免交叉污染。此外,TissueRuptor使用一次性透明钻头,可以直观的监控样本破碎过程。

应用

TissueRuptor可高效破碎并匀浆人类、植物和动物组织样本,包括血块。使用QIAGEN试剂盒对RNA、DNA、总核酸或蛋白质进行纯化。

辅助数据和图表

Specifications

FeaturesSpecifications
Disruption principleSimultaneous disruption and homogenization by rotation of the blade of the disposable probe
TechnologyHandheld rotor-stator homogenizer
Kits compatible with instrumentAll Kits for purification of RNA/DNA/protein
Technical dataEU / UK : 230V AC 125W 10,000 – 33,000rpm; US:120V ~ 1.15A 60Hz 5,000 – 35,000rpm
Typical run time15–120 seconds
FeaturesRapid, effective disruption of a range of sample types. Disposable probes minimize risk of cross-contamination. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies.
Throughput1 sample per run

资源

Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

2. Can the TissueRuptor stainless steel probe be autoclaved?

Yes, the stainless steel probe can be autoclaved.

FAQ ID -9141
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?

Yield and integrity of genomic DNA depend on the disruption and homogenization method used with the Allprep DNA/RNA Kits.

Homogenization with the TissueRuptor (or other rotor–stator homogenizer, such as the Polytron) or the TissueLyser results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, so DNA yields will be higher.

In contast, homogenization using the QIAshredder (or a syringe and needle) is more gentle, resulting in less DNA fragmentation. However, longer DNA fragments are more difficult to elute, so DNA yields may be lower.

 

FAQ ID -1751
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.  

FAQ ID -374
What is the minimum liquid volume that should be used with the TissueRuptor
The minimum volume that should be used is 180 µl in a suitably sized vessel (e.g., 2 ml microcentrifuge tube).
FAQ ID -9144
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?

We recommend using either the TissueRuptor or the TissueLyser for homogenization of tissues stored in Allprotect Tissue Reagent.

Since tissues incubated in Allprotect Tissue Reagent are harder than fresh tissues, longer homogenization times might be necessary (for details, see the Allprotect Tissue Reagent Handbook).

Furthermore, we do not recommend using rotor–stator homogenizers with a metal probe (e.g., Polytron) if proteins will be purified subsequently, as the metal probe can heat the sample.

 

FAQ ID -1572
Can I use Buffer ATL for disruption of my samples with TissueRuptor, TissueLyser II, or TissueLyser LT instruments?

Buffer ATL can produce foam when used in conjunction with the TissueRuptor. You can add 0.5% final volume of Reagent DX (cat. no. 19088) to prevent the formation of foam.

FAQ ID -9143
3. Can the TissueRuptor instrument be autoclaved?

We do not recommend to autoclave any part of the TissueRuptor, except the probe adapter for disposable probes.

FAQ ID -9142
Can the TissueRuptor Disposable Probes be autoclaved?

Disposable probes can be autoclaved up to 5 times (15 min, 120°C).

FAQ ID -9140
Can the disposable probes be re-used? How should they be washed?
We do not recommend using the probes more than 5 times. Several options for sterilizing and decontaminating the probes can be found in the TissueRuptor User Manual and TissueRuptor Handbook.
FAQ ID -9146
What is the catalog number for steel probes and disposable probes for use with QIAGEN’s TissueRuptor instrument? What are the dimensions of these probes?
  • TissueRuptor Disposable Probes (catalog no. 990890) have a diameter of 7 mm (0.3 in) and are 110 mm (4.3 in) long. They are supplied as a pack of 25.
  • TissueRuptor Steel Probe (catalog no. 9017341) has a diameter of 7 mm (0.3 in) and are 85 mm (3.3 in) long. They are supplied as 1 per pack.

To place an order or to enquire pricing, please contact QIAGEN Customer Care.

FAQ ID -9145