EZ1 Virus Mini Kit v2.0

每批可从1–6个或1–14个样本中自动纯化病毒核酸

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EZ1&2 Virus Mini Kit v2.0 (48)

Cat. No. / ID: 955134

For 48 preps: Reagent Cartridges (Virus Mini v2.0), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer AVE, carrier RNA

JP¥46,500

The name of this kit was changed from "EZ1 Virus Mini Kit v2.0" to "EZ1&2 Virus Mini Kit v2.0" to demonstrate that it is compatible with the new EZ2 Connect instruments as well as the EZ1 instruments. No changes were made to the well-established kit technology.

EZ1 Virus Mini Kit v2.0 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

同时纯化病毒DNA和RNA
更高的灵敏度，适用于低滴度病毒
高重复性结果
针对宽浓度范围的病毒滴度，产量线性契合度高
预封装的试剂条，操作方便

产品详情

EZ1 Virus Mini Kit v2.0提供全自动操作方法从血清、血浆、脑脊液、尿液、呼吸道样本和其他无细胞体液中同时纯化病毒DNA和RNA，用于敏感的下游应用。经优化的结合条件，确保获得更完整的核酸；经优化的洗涤条件，使纯化获得的核酸适用于敏感的下游分析。该试剂盒配合EZ1 Advanced XL（每批处理1–14个样本）、EZ1 Advanced（每批处理1–6个样本）和BioRobot EZ1（每批处理1–6个样本）工作站使用。

原理

使用EZ1 Virus Mini Kit v2.0进行自动纯化需使用EZ1 Advanced XL和EZ1 Advanced XL Virus Card v2.0、EZ1 Advanced和EZ1 Advanced Virus Card v2.0或BioRobot EZ1和EZ1 Virus Card v2.0。EZ1卡预装有纯化程序，在控制面板提供操作向导，并提供EZ1 Advanced工作站的运行指令。

使用硅胶膜磁珠技术，一次操作可纯化1–14个样本（EZ1 Advanced XL）或1–6个样本（EZ1 Advanced和BioRobot EZ1）。预封装的试剂条和仪器的自动感控锁可降低污染的风险，避免RNase导致的核酸降解。

程序

将血清或血浆样本（100 µl、200 µl或400 µl）上样至工作站中，全自动EZ1 Virus纯化操作后，DNA被一步纯化。病毒DNA洗脱体积为150 µl、120 µl、90 µl或60 µl，可直接用于各种下游应用。
该方法纯化病毒核酸方便可靠。EZ1 Virus Mini Kit v2.0采用预封装试剂条，减少人工操作的错误；EZ1 Cards含有纯化方案，无需手动输入数据。1–6个样本的全自动纯化只需40分钟，包括仅仅5分钟的手动操作时间。

Specifications

Features	Specifications
Applications	PCR, real-time RT-PCR
Elution volume	50–200 µl
Technology	Magnetic particles
For automated processing	BioRobot EZ1
Sample amount	100 µl, 200 µl, 400 µl
Processing	Automated
Format	Sample tube
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Viral DNA, viral RNA
Time per run or per prep	40 minutes
Main sample type	Serum, plasma

资源

For use with EZ2 Connect instruments

EZ1&2 Virus Mini Kit v2.0
For use with EZ2 Connect instruments

EZ1&2 Virus Mini Kit v2.0
For automated, simultaneous purification of viral DNA and RNA, and bacterial DNA using EZ2® Connect instruments

EZ1&2 Virus Mini Kit v2.0
For automated, simultaneous purification of viral DNA and RNA, and bacterial DNA, from 1-14 samples of serum, plasma, and CSF, urine, whole blood, and stool, transport media, respiratory samples, and dried swab samples using EZ1 instruments

Fully automated low- to medium-throughput purification of nucleic acids

Download Safety Data Sheets for QIAGEN product components.

FAQ

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246