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QIAGEN Plasmid Plus 96 Miniprep Kit (4)
Cat. No. / ID: 16181
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well blocks.
用于用于仪器
QIAvac 96
BioRobot Universal
QIAGEN Plasmid Plus 96 Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
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特点
- 纯化得到转染级质粒DNA，内毒素水平<1 EU/µg DNA
- 产物DNA的纯度可与阴离子交换方法得到的纯度相同
- 高产量（至多50 μg）
- 操作便利、流程简单
- 提供真空、离心和自动化处理操作方法
产品详情
QIAGEN Plasmid Plus 96 Kits提供创新的方法通过96孔板纯化质粒。样本可在BioRobot Universal System上通过QIAvac 96和/或离心机处理或自动运行。使用专有的结合试剂，确保从5 ml E. coli培养液中每孔可纯化获得至多50 μg转染级质粒DNA。独特的内毒素去除缓冲液可使内毒素水平低至1 EU/μg DNA。
绩效
即用型质粒DNA，产量高且稳定
QIAGEN Plasmid Plus技术可达到与阴离子交换技术相同的性能和品质。可纯化获得多达50 μg的转染级质粒DNA（参见"High yields of plasmid DNA"）。
极低的内毒素水平
该试剂盒应用内毒素去除缓冲液可有效降低内毒素水平至1 EU/μg DNA，得到的质粒DNA适合在敏感细胞系中转染（参见"Very low endotoxin levels"和"Highly efficient transfection into a sensitive cell line"）。
原理
QIAGEN Plasmid Plus 96 Kits基于QIAGEN Plasmid Plus技术，提供纯化质粒的创新方法。该操作流程基于非离液盐结合试剂，选择性好，且获得的DNA的品质与采用阴离子交换技术。裂解物澄清后，只需简单的结合-洗涤-洗脱过程，就可得到高浓度的质粒DNA，可直接用于下游应用。独特的试剂和Plasmid Plus 96板的设计确保了高效的结合能力。
程序
应用96孔板纯化质粒这种创新方法，只需简单的结合-洗涤-洗脱过程，就可得到高纯度转染级质粒DNA，（参见"QIAGEN Plasmid Plus 96 procedure"）。
QIAGEN Plasmid Plus 96 Miniprep首先采用碱性裂解法，中和后，裂解物使用TurboFilter 96孔板澄清。澄清后的裂解物中加入非离液盐结合缓冲液确保质粒DNA充分与Plasmid Plus 96孔板的膜进行结合。独特的结合缓冲液可确保获得的DNA品质相当于采用阴离子交换技术制备的质粒DNA。内毒素去除缓冲液洗涤后，内毒素水平低至1 EU/μg DNA， 得到的DNA适合在敏感细胞系中转染。QIAGEN Plasmid Plus 96 Miniprep专有的结合试剂可从5 ml E. coli培养液中每孔可纯化获得多达50 μg转染级质粒DNA。
方便、灵活的操作选择
可在BioRobot Universal System上通过QIAGEN Plasmid Plus 96 BioRobot Kit进行自动纯化。也可将QIAvac 96与TurboFilter 96孔板配套使用，清洗裂解物，随后在Plasmid Plus 96板上进行DNA结合和洗涤。然后在QIAvac 96或兼容的离心机上进行烘干和洗脱。此外，整个流程都可在兼容的离心机上进行。
应用
QIAGEN Plasmid Plus 96 Kits纯化得到的质粒DNA，适合多种应用，包括：
- 转染，包括敏感细胞系转染
- 制备短发夹载体（sh载体）
- 酶修饰
- 克隆
- 限制性酶切
辅助数据和图表
Very low endotoxin levels.
pCMVβ plasmid DNA was purified from 1.3 ml and 5 ml E. coli DH10B cultures using the QIAGEN Plasmid Plus 96 Miniprep Kit, the QIAwell 96 Ultra Plasmid Kit, and kits from other suppliers. Endotoxin levels were determined using the Kinetic-QCL Kinetic Chromogenic Assay. For the EndoFree Plasmid Giga Kit, the endotoxin content was 0.0005 EU/μg DNA. EF: EndoFree Plasmid Giga Kit. QPP96: QIAGEN Plasmid Plus 96 Miniprep Kit. QW96: QIAwell 96 Ultra Plasmid Kit.
Specifications
| Features | Specifications |
|---|---|
| Applications | Fluorescent and radioactive sequencing, cloning etc. |
| Time per run or prep per run | <70 minutes |
| Processing | Automated |
| Culture volume/starting material | 1–10 ml culture volume |
| Technology | Silica technology |
| Yield | <20 µg |
| Samples per run (throughput) | 48–96 samples per run |
| Plasmid type | High-copy, low-copy, cosmid DNA |
资源
试剂盒操作手册 (2)
快速启动实验方案 (1)
产品介绍与指南 (1)
安全数据表 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?