QIAvac Accessories

与QIAvac真空底座配合使用

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QIAvac Holder

Cat. No. / ID: 19418

Holder for stabilizing QIAGEN Plasmid Plus Mega and Giga columns with Tube Extenders on the QIAvac 24 Plus

附件产品类型

QIAvac Holder

QIAvac Luer Adapter Set

VacConnectors

VacValves

Vacuum Pump

Vacuum Regulator

Vacuum Pump (230 V, 50 Hz)

Inquire

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

提高使用便利性

产品详情

QIAvac Accessories是用于辅助96孔板或离心柱操作的配件。

绩效

QIAvac Vacuum Manifolds是真空底座，协助QIAvac多孔模块或离心柱的样本处理。

推荐使用的QIAvac Accessories
规格	试剂盒	QIAvac model	配件
96孔板	QIAGEN Plasmid Plus 96, QIAprep 96 Plus, QIAprep 96 Turbo, QIAquick 96, R.E.A.L. 96, RNeasy 96	QIAvac 96	Vacuum Regulator （可选）
96孔板	DirectPrep 96 MiniPrep Kit, MinElute 96 UF PCR Purification Kit	QIAvac Multiwell	–
离心柱	QIAprep, MinElute, QIAquick, RNeasy, QIAGEN Plasmid Plus Midi, QIAGEN Plasmid Plus Maxi, CompactPrep Midi, CompactPrep Maxi	QIAvac 24 Plus	–
		或
		QIAvac 6S*	QIAvac Luer Adapters
	QIAamp Mini （适合真空实验方案的试剂盒）	QIAvac 24 Plus	VacConnectors VacValves（可选）
		o或
		QIAvac 6S*	QIAvac Luer Adapters VacConnectors
	QIAamp Midi, QIAamp Maxi, QIAamp DSP^†	QIAvac 24 Plus	VacConnectors VacValves （可选） QIAvac Connecting System
	QIAGEN Plasmid Plus Giga, QIAGEN Plasmid Plus Mega	QIAvac 24 Plus	QIAvac Holder

原理

QIAGEN Vacuum Pump适用于实验室标准真空操作（34 L分钟, 8 mbar）。Vacuum Pump配套QIAvac真空底座，可真空处理QIAGEN的多种核酸纯化试剂盒。在真空的作用下，液体流过，可替代离心操作，减少样本制备所需的手动操作时间。

QIAvac Holder（参见" QIAvac Holder"）可与Tube Extenders用在QIAvac 24 Plus上，放置QIAGEN Plasmid Plus Mega and Giga离心柱，防止离心柱晃动，尤其在离心柱含有大体积液体时。

VacValves可直接插在QIAvac 24 Plus底座的鲁尔接头上，应在样本流速有显著差异时使用。在处理粘稠样本，如血液时，应使用VacValves以确保真空流速均匀。

VacConnectors（参见" VacConnector"）是一次性接头，可装在QIAamp离心柱或QIAprecipitator模块的外管口，也可接在QIAvac 24、QIAvac 6S（已停产）的QIAvac Luer Adapters或QIAvac 24的VacValves的鲁尔接头上。该配件可代替离心，用于分离核酸。VacConnectors可防止在纯化过程中离心柱或模块与鲁尔接头直接接触，避免产生任何交叉污染。VacConnectors为一次性产品，使用后应丢弃。

QIAvac Luer Adapters可在QIAvac 6S（已停产）上便利的处理至多24个离心柱。在真空作用下，样本和洗涤液流过离心柱，因此无需离心，加快了纯化过程。QIAprep、QIAquick和MinElute离心柱的接口可直接插在QIAvac Luer Adapters的鲁尔接头上。每个Luer Adapter含有4个鲁尔接头（参见照片），QIAvac 6S上层板上可连接6个Luer Adapters，因此一次可处理24个样本。QIAamp离心柱在QIAvac 6S（已停产）上使用时需配套使用VacConnectors和QIAvac Luer Adapters。

Vacuum Regulator（参见" Vacuum Regulator"）可便利的监控并调整使用QIAvac底座时的真空压。QIAGEN纯化试剂要求真空压在一定范围内（如产品使用手册中所写），这样才能获得理想产量和理想纯度的核酸。

查看图表

QIAvac Holder.

VacConnectors.

Vacuum Regulator.

程序

实验流程请参照相关产品使用手册中的指导说明。

应用

QIAvac Accessories用于样本的真空处理，适合多种应用。

辅助数据和图表

QIAvac Holder.

QIAvac Holder with QIAvac 24 Plus and QIAGEN Plamid Plus Mega and Giga Columns with Tube Extenders.

资源

Download Safety Data Sheets for QIAGEN product components.

The procedure has been used successfully for isolation of a single-copy, 14.5 kb, binary plasmid, p35S GUS INT, from Agrobacterium tumefaciens strain GV2260 (1).

This procedure has been adapted by customers and is for purification of viral RNA and DNA from plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit.

FAQ

We do not recommend using a vacuum manifold for DNA extraction using the QIAamp 96 DNA Blood Kit because:

Viscosity of the lysates varies from sample to sample. Therefore, the wells of the QIAamp 96 plate are at great risk of clogging.
Use of vacuum manifold would result in incomplete removal of ethanol and salts after washing with Buffer AW2. This will affect the purity and yield of samples.

FAQ ID -9158

Please contact Nalgene for ordering. Part numbers from Nalgene:

- 10 liter waste container (Nalgene Heavy Duty Vacuum Carboy [PP], cat. no. 2226-0020)

- 20 liter waste container (Nalgene Heavy Duty Vacuum Carboy [PP], cat. no. 2226-0050)

- Quick filling/venting closure (Nalgene, cat. no. 2158-0021) [lid]

- Vacuum tubing (e.g., Nalgene 180 clear plastic vacuum tubing, cat. no.8000-0065)

FAQ ID -9156

It is possible to use a general laboratory vacuum system with the QIAamp DSP Virus Kit. However, this will require validation in-house. The CE-marked QIAamp Kits have been developed, field-tested and validated with the QIAvac 24 Plus vacuum system (QIAvac 24 Plus, QIAvac Connecting System and Vacuum Pump). Using this system with the QIAamp DSP Virus Kit will avoid lengthy validation procedures for the enduser.

FAQ ID -789

VacConnectors prevent cross-contamination and are meant to be disposed after single use.

FAQ ID -9152

Yes, the waste bottles of the QIAvac Connecting System can be autoclaved. However, please be aware that the plastic may change in color.

FAQ ID -9153

We do not recommend using a vacuum manifold for DNA extraction of tissues using the DNeasy Blood & Tissue Kit. Viscosity of the lysates varies from sample to sample; therefore, the DNeasy mini spin columns are at great risk of clogging.

FAQ ID -9154

We do not recommend to autoclave the QIAvac 24 Plus manifold. Doing so can result in damage to the device.

FAQ ID -9151

We do not recommend using a vacuum manifold for DNA extraction with the DNeasy 96 Blood & Tissue Kit because:

Viscosity of the lysates varies from sample to sample. Therefore, the wells of the DNeasy 96 plate are at great risk of clogging.
Use of vacuum manifold would result in incomplete removal of ethanol and salts after washing with Buffer AW2. This will affect the purity and yield of samples.

FAQ ID -9157

QIAvac Multiwell can hold plates that conform to SBS plate standards, whereas the QIAvac 96 does not. Therefore, the waste tray for QIAvac Multiwell is not the same as for our QIAvac 96.

However, the waste tray from QIAvac Multiwell will fit the QIAvac 96, but not vice versa.

FAQ ID -9159

We do not have this part available for replacement. You will need to replace your vacuum manifold. Please contact your Sales representative for a price quote.

FAQ ID -9161

FAQ ID -9160

We do not recommend using a vacuum manifold for DNA extraction of tissues using the QIAamp Blood & Tissue Kit. Viscosity of the lysates varies from sample to sample; therefore, the DNeasy mini spin columns are at great risk of clogging.

FAQ ID -9155

The filter for QIAvac Connecting System is from Pall (part no. 4400). This is the Acro 50 filter, 0.2 µm PTFE, 1/8 inch MNPT connection. Please visit www.pall.com.

FAQ ID -9150