artus HSV-1/2 PCR Kits CE

应用real-time PCR对HSV-1/2的DNA进行检测和分型

Products

artus HSV-1/2 PCR Kits CE 旨在用于欧洲体外诊断用途。

artus HSV-1/2 LC PCR Kit (96) CE

Cat. No. / ID: 4500065

For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, Mg Solution, 4 Quantitation Standards (HSV-1), 4 Quantitation Standards (HSV-2), Internal Control, Water (PCR grade)

artus HSV-1/2 RG PCR Kit (24) CE

Cat. No. / ID: 4500263

For 24 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 2 Positive Controls, Internal Control, Water (PCR grade)

artus HSV-1/2 TM PCR Kit (96) CE

Cat. No. / ID: 4500165

For 96 reactions on the ABI PRISM 7000 and 7900HT SDS: Master, 4 Quantitation Standards (HSV-1), 4 Quantitation Standards (HSV-2), Internal Control, Water (PCR grade)

artus HSV-1/2 LC PCR Kit (24) CE

Cat. No. / ID: 4500063

For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, Mg Solution, 4 Quantitation Standards (HSV-1), 4 Quantitation Standards (HSV-2), Internal Control, Water (PCR grade)

artus HSV-1/2 QS-RGQ Kit (24) CE

Cat. No. / ID: 4500363

For 24 reactions on the QIAsymphony RGQ: Master, Mg Solution, 2 Positive Controls, Internal Control, Water (PCR grade)

artus HSV-1/2 RG PCR Kit (96) CE

Cat. No. / ID: 4500265

For 96 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 2 Positive Controls, Internal Control, Water (PCR grade)

artus HSV-1/2 TM PCR Kit (24) CE

Cat. No. / ID: 4500163

For 24 reactions on the ABI PRISM 7000 and 7900HT SDS: Master, 4 Quantitation Standards (HSV-1), 4 Quantitation Standards (HSV-2), Internal Control, Water (PCR grade)

特点

工作流程完全符合体外诊断CE认证（QIAsymphony RGQ）
使用内参和阳性对照，具有高度可靠性
灵敏度高，可达57.3拷贝/ml（QS-RGQ试剂盒）
检测多种病毒的温度条件相同（仅适用于RG和QS-RGQ试剂盒）

产品详情

artus HSV-1/2 PCR Kits是使用real-time PCR技术的即用型分子检测试剂盒。该试剂盒包含体外诊断中对脑脊液（(RG和QS-RGQ试剂盒）、血浆、血清和拭子（LC和TM试剂盒）中纯化的HSV-1/2 DNA进行快速、灵敏检测的所有所需试剂，并已经过优化。artus HSV-1/2 LC、RG和TM PCR Kits使用自动或手动样本制备和手动反应体系构建技术。artus HSV-1/2 RG PCR Kit另有EASYartus HSV-1/2 RG PCR Kits，可与用于自动纯化病毒核酸的EZ1 DSP Virus Kit配合使用。artus HSV-1/2 QS-RGQ Kit可在QIAsymphony RGQ仪器上使用，从样本制备到病原体检测均为完全自动完成，不可进行手动反应体系构建。

绩效

为确保高灵敏度，artus HSV-1/2 Kits已经过优化，可检测到低拷贝数的HSV-1/2 RNA。artus HSV-1/2 QS-RGQ Kit在QIAsymphony RGQ上使用时，对HSV-1的分析灵敏度为57.3拷贝/ml，对HSV-2的分析灵敏度为65.7拷贝/ml（参见" Highly sensitive detection of HSV-1 DNA"）。

*artus* HSV-1/2 RG PCR Kit和*artus* HSV-1/2 QS-RGQ Kit的性能特征
试剂盒	artus HSV-1/2 RG PCR Kit	artus HSV-1/2 QS-RGQ Kit
经过验证的使用样本类型	脑脊液	脑脊液
分析灵敏度	HSV-1为0.12拷贝/μl HSV-2为0.16拷贝/μl	HSV-1为57.3拷贝/ml HSV-2为65.7拷贝/ml
特异性	已使用HSV-1的HF、KOS、MacIntyre株系以及HSV-2的HG-52、G和MS株系进行验证	已使用HSV-1的HF、KOS、MacIntyre株系以及HSV-2的HG-52、G和MS株系进行验证

查看图表

Highly sensitive detection of HSV-1 DNA.

原理

artus HSV-1/2 PCR Kits的原理为使用real-time PCR技术对HSV-1和HSV-2基因组特定片段进行扩增和实时检测。该试剂盒特异性强、灵敏度高（参见" Highly sensitive detection of HSV-1 DNA"），且重复性好。

每个试剂盒中都包含HSV-1和HSV-2的阳性对照（参见" Detection of the HSV-1 positive control"和" Detection of the HSV-2 positive control"）。此外，该试剂盒还含有异源扩增系统，即内参，可识别PCR抑制剂。内参（IC）可在PCR中通过不同的荧光通道检测。

*artus* HSV-1/2 RG PCR Kit和*artus* HSV-1/2 QS-RGQ Kit的规格
试剂盒	artus HSV-1/2 RG PCR Kit和artus HSV-1/2 QS-RGQ Kit
经过验证的适用样本类型	脑脊液
扩增子	UL5基因的一段长为154 bp的序列

查看图表

Highly sensitive detection of HSV-1 DNA.

Detection of the HSV-1 positive control.

Detection of the HSV-2 positive control.

程序

artus HSV-1/2 PCR Kits包含对HSV-1/2 DNA进行可靠检测和分型的所有所需试剂，并已经过优化。只需在即用型PCR预混液和Mg溶液中加入模板DNA，即可按照试剂盒操作手册中的优化程序，在real-time PCR仪上开始反应。

多个试剂盒的反应温度相近，一次检测可进行多个分析

artus HSV-1/2 LC PCR, RG PCR, and QS-RGQ Kits的反应温度与artus LC PCR、RG PCR和QS-RGQ Kits检测EBV、CMV、VZV和BK病毒的温度相应。因此可一次进行多个PCR分析。

完整的自动化检测体系，覆盖样本制备到HSV-1/2检测

QIAsymphony RGQ的HSV-1/2检测解决方案包括：使用QIAsymphony SP进行样本制备，使用QIAsymphony AS进行反应体系构建，并在Rotor-Gene Q实时荧光定量PCR分析仪上使用artus HSV-1/2 QS-RGQ Kit 进行检测。这一体系不仅可可靠检测病原体，而且完全符合体外诊断的CE要求（参见" Integrated QIAsymphony RGQ system for HSV-1/2 detection"）。

如使用artus LC、RG和TM PCR Kits，推荐用以下产品纯化病毒DNA

可使用EZ1 DSP Virus Kit自动纯化病毒DNA。artus HSV-1/2 RG PCR Kit还有经体外诊断CE认证的EASYartus HSV-1/2 RG PCR Kits，可与用于自动纯化病毒核酸的EZ1 DSP Virus Kit共同使用。

查看图表

Integrated QIAsymphony RGQ system for HSV-1/2 detection.

应用

artus HSV-1/2 PCR Kits可快速、灵敏的检测EDTA血浆中的HSV-1/2 DNA。这些试剂盒可利用基因分型特异性探针对HSV-1和HSV-2进行分型。

artus HSV-1/2 QS-RGQ Kit专为QIAsymphony RGQ设计。从样本制备到HSV DNA的检测的整个工作流程符合体外诊断CE认证的要求，并且可对HSV-1和HSV-2进行分型。

辅助数据和图表

Detection of the HSV-1 positive control.

HSV-1 RG PC is detected in fluorescence channel Cycling Green. NTC: No template control (negative control).

Specifications

Features	Specifications
Quantitative/qualitative	Quantitative/qualitative
Sample type	Serum, plasma, cerebrospinal fluid (CSF), swabs
RUO/CE/ASR	CE
Recommended sample prep	QIAamp DNA Mini Kit, QIAamp UltraSens Virus Kit, EZ1 DSP Virus Kit
Thermal cycler	ABI PRISM 7000, 7900HT SDS, LightCycler, Rotor-Gene Q Instruments
What detected	Herpes Simplex Virus 1 and 2 DNA

资源

Information document for software version 4.0

For use with QIAsymphony SP/AS instruments (software version 4.0)

Download Safety Data Sheets for QIAGEN product components.

FAQ

In general and especially for extraction from cerebrospinal fluid, serum or plasma, QIAGEN recommends the use of carrier RNA during nucleic acid extractions to increase the yield of extracted pathogen DNA. QIAGEN recommends to use Poly(A) RNA homopolymers from GE Healthcare (Cat. no. 27- 4110-02).

FAQ ID -1530

The artus HSV-1/2 RG PCR kit detects HSV-1/2 specific DNA. Other Herpes viruses are not detected. The LOD is 0.12 copies/µl for HSV-1 and 0.16 copies/µl for HSV-2

-1

Only CSF is validated within the CE mark for the artus HSV-1/2 RG PCR Kit.

-1

No. The artus HSV-1/2 RG PCR Kit is highly specific and shows no cross-reactivities. Sample materials with the following pathogens tested negative: EBV, VZV, CMV, HHV6, HAV, HBV, HCV, HIV, WNV, Parvo B19, Enterovirus.

-1

The artus^® HSV-1/2 LC PCR Kit contains probes for both the HSV-1 and HSV-2 detection. Yet, the hybridization properties of these probes differ with the template DNA (HSV-1 or -2) to allow for a melting curve based virus type discrimination. The probes which match HSV-1 perfectly carry a few mismatches for HSV-2 leading to decreased fluorescence signal.

-2