Investigator IDplex Plus Kit

用于多重扩增 13 个 CODIS 核心基因座，外加 D2S1338、D19S433 和釉原蛋白基因，也用于直接从拭子和纸张进行多重扩增

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Investigator IDplex Plus Kit (100)

Cat. No. / ID: 381625

引物混合液、含 HotStarTaq Plus DNA 聚合酶的快速反应混合液、对照 DNA、等位基因分型标准物 IDplex Plus、DNA 分子量标准物 550 (BTO) 和无核酸酶水

KitAccessories

Investigator IDplex Plus

Matrix Standard

Reactions

100

400

Inquire

Investigator IDplex Kit 适用于法医鉴定、人类身份识别和亲子鉴定的分子生物学领域。这些产品不能用于疾病诊断、预防和治疗。这些产品并非在所有国家/地区都上市销售。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

同时扩增 16 个基因座，包括 CODIS 核心基因座
非常适合法医鉴定案件或参考样本
采用即用型 Fast Reaction Mix 的简单方案
优化了引物设计以减少基因座重叠

产品详情

Investigator IDplex Plus Kit 是一种用于人类身份确定的多重 STR 检测试剂盒，涵盖 13 个 CODIS（DNA 联合索引系统）核心基因座、D2S1338、D19S433 和釉原蛋白基因。

该试剂盒利用我们的快速循环技术，可实现在大约 90 分钟内完成扩增。Investigator IDplex Plus Kit 具有高度灵敏度，可从痕量 DNA 中快速获得可靠的结果，适用于所有法医应用和亲子鉴定。基因分析仪使用 BT5 Matrix 进行校准。

Investigator IDplex Kit 符合 ISO 18385 要求。

绩效

Investigator IDplex Kit 利用快速循环技术进行 STR 检测，在大约 90 分钟内便可从提取的 DNA 中获得可靠的结果。

Fast Reaction Mix 基于久经考验的 QIAGEN 多重 PCR 技术，并对该技术进行优化，可以增强抑制物耐受性。在 6 种类型抑制物（腐殖酸、血红素、鞣酸、靛蓝胭脂红、钙和胶原蛋白）浓度增加的情况下测试的检测性能显示出高灵敏度和优异的抑制物耐受性。这使得能够从案件样本中获得可靠的高品质结果。

使用 GeneAmp 9700 热循环仪和 Applied Biosystems 3500 Genetic Analyzer 对 Investigator IDplex Kit 进行验证和评价。

在标准条件下，Investigator IDplex Plus Kit 的 DNA 推荐用量为 0.5 ng。内部验证已证实使用 0.2–2 ng DNA 可获得稳定、均衡的结果，使用 <0.1 ng DNA 可获得可靠的结果。

原理

Investigator IDplex Kit 使用 QIAGEN 的快速循环 PCR 技术同时对 13 个 CODIS（DNA 联合索引系统）核心基因座、D2S1338、D19S433 和釉原蛋白基因进行快速扩增。精心设计的引物减少了基因座等位基因的重叠，从而最大程度地减少了误读的可能性。

程序

15 种多态性高的常染色体 STR 基因座（D2S1338、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D19S433、D21S11、CSF1PO、FGA、TH01、TPOX 和 vWA）以及性别特异性釉原蛋白基因在单一 PCR 中同时扩增，并用“Investigator IDplex Plus Kit 染料及对应标志物”表中列出的某种染料进行标示。

Investigator IDplex Plus Kit 染料及对应标志物
染料	基因座
6-FAM	Amelogenin	TH01	D3S1358	vWA	D21S11
BTG	TPOX	D7S820	D19S433	D5S818	D2S1338
BTY	D16S539	CSF1PO	D13S317	FGA
BTR	D18S51	D8S1179

然后对扩增的基因座进行毛细管电泳。可以使用 GeneMapper ID-X 等专用软件分析结果。有关详细信息，请参阅“技术规格”表。

应用

Investigator IDplex Plus Kit 用于从血液、口腔拭子和法医学斑迹或参考样本中快速可靠地生成 DNA 图谱，非常适合：

犯罪数据库、法医参考或案件样本
亲子鉴定
群体遗传学研究
其他人类身份确定检测应用

技术规格
特点	Investigator IDplex Plus Kit
DNA 输入体积	最多 15 µl
每次反应的容量	25 µl
矩阵	BT5
荧光染料	6-FAM、BTG、BTY、BTO
基因分析仪	ABI PRISM 3100/3100 Avant、Applied Biosystems 3130/3130xl 或 Applied Biosystems 3500 Genetic Analyzer
分析软件	Applied Biosystems GeneMapper ID 或 GeneMapper ID-X

资源

Advanced sample collection, automation and STR solutions for kinship testing

For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.

The most comprehensive and versatile coverage of current international STR standards for capillary electrophoresis analysis

Quality initiatives for human identity testing and forensics

Download Safety Data Sheets for QIAGEN product components.

Advanced sample collection, automation and STR solutions for kinship testing

The most comprehensive and versatile coverage of current international STR standards for capillary electrophoresis analysis

Quality initiatives for human identity testing and forensics

For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.

FAQ

Yes. The Fast Reaction Mix of the Investigator IDplex Plus Kit contains HotStarTaq Plus enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only Investigator IDplex Plus Primer Mix and template DNA need to be added. Nuclease-free Water should be added to complete the reaction volume.

FAQ ID -3008

We recommend first adding master mix to the PCR plate and then adding the punch to avoid punches getting lost or mixed up due to electrostatic effects. Furthermore, we strongly recommend spinning down the PCR plate after setup to ensure all punches get fully submerged.

FAQ ID -3096

Yes. The protocol is generic and has successfully been tested on various commonly used instruments.

FAQ ID -3089

Yes. The protocol of the Investigator IDplex Plus Kit is generic and has been successfully tested on various commonly used instruments.

FAQ ID -3009

15 µl input volume can be used with the Investigator IDplex Plus Kit.

FAQ ID -3014

Amplification of Control DNA 9948 is appropriate as a PCR performance check. However, if you have access to reference samples at constant quality (for example, swabs or FTA donated by lab personnel), these samples may be used as additional in process controls.

FAQ ID -3094

If you are using an ABI PRISM 3100-Avant/3100 or 3130/3130xl Genetic Analyzer, please use Any5Dye, if available. If you are using the Applied Biosystems 3500/3500xL Genetic Analyzer, please use filter set AnyDye. Otherwise, filter set G5 should be used. If you are using an ABI PRISM 310 Genetic Analyzer, filter set G5 should be used.

FAQ ID -3017

No. Unlike some other QIAGEN STR products (e.g., the Investigator Argus Y-12 QS Kit) there is no QS quality sensor included in the Investigator IDplex Plus Kit.

FAQ ID -3011

No. Up to 2 µl can be added on top of a 25 µl reaction.

FAQ ID -3088

We do not recommend using more than one punch of 1.2 mm diameter for blood or buccal cells on FTA, or 2 µl of a crude buccal swab lysate. To adapt protocols to the type and quality of samples used in your laboratory, and to the CE instrumentation in use, adjust PCR cycle numbers and CE injection conditions.

FAQ ID -3090

Yes. Use of indicator cards helps to ensure sample is taken from an appropriate area of the FTA card. This may significantly improve success rates compared to procedures using non-indicator cards.

FAQ ID -3095

The optimum sample DNA input range of the Investigator IDplex Plus Kit is between 200–500 pg. However, reliable results can be obtained with less than 100 pg. For higher amounts of input DNA, consider adjusting the PCR cycle number.

FAQ ID -3015

No. Amplification products of marker FGA have a slightly different size between IDplex Plus and IDplex GO! kits due to a change in the fluorescent dye linker used. Use of the IDplex Plus ladder will frequently cause off ladder calls for FGA alleles.

FAQ ID -3093

Yes. The Fast Reaction Mix contains Taq enzyme, Mg²⁺, and dNTPs in a pre-optimized concentration. Only IDplex GO! Primer Mix and sample needs to be added.

FAQ ID -3087

Yes. Sample materials used for direct amplification vary with regard to quality and quantity of DNA present, depending on sampling materials, procedures, and storage conditions. In order to obtain the highest possible success rates in routine use, it is essential to run a representative batch of samples for evaluation of optimal conditions. Furthermore, we recommend revisiting this aspect after a certain period of routine use to ensure the evaluated conditions are optimal and re-adjust if necessary.

FAQ ID -3091

No, the primer site locations of the markers in the Investigator IDplex Plus Kit have not been changed. Only minor changes have been introduced for some markers, e.g., to improve A addition to the PCR products.

FAQ ID -3012

Matrix BT5 should be used with Investigator IDplex Plus Kit.

FAQ ID -3016

The BT5 matrix standard is designed for use with our 5-dye Investigator STR Assays. We do not disclose the containing dyes.

FAQ ID - 3364

Yes. For example, cycle numbers can be increased to 32 or 34 in order to obtain higher signals for very diluted samples. However, please note that this promotes the amplification of nonspecific products or amplification from spurious background DNA.

FAQ ID -3010

The Investigator IDplex Plus Kit contains Primer mix, Fast Reaction Mix, Control DNA allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water.

FAQ ID -3013

The template files for the Investigator IDplex Plus Kit for various softwares and analyzers can be downloaded from the Resource tab on the product webpage.

FAQ ID -3019

No, primer site locations of markers have not been changed. Two additional SNP primers have been added to allow balanced amplification of vWA and D16S539 alleles carrying known primer binding site mutations. The mutations covered were observed in concordance studies in samples of African origin at an elevated level. The labelled FGA forward primer has been slightly changed with regard to the linker of the fluorescent dye. The change does not affect PCR amplification, but causes a slight size shift during capillary electrophoresis. The allelic ladder therefore has been changed accordingly.

FAQ ID -3092

For Any5Dye filter set with the Investigator IDplex Plus Kit, please use:

Condition Bound: Lower 1.0 – Upper 20
Sensitivity: 0.1
Quality Value: 0.80

FAQ ID -3018