QIAamp DSP Circulating NA Kit

用于从人类样本中浓缩和纯化游离DNA、RNA和miRNA

特点

浓缩核酸；上样量达，洗脱体积小
高效回收片段化DNA、RNA和miRNA
无需有机抽提和乙醇沉淀
去除污染物和抑制剂

产品详情

QIAamp DSP Circulating NA Kit用于从血浆和血清中浓缩和纯化游离DNA和RNA。

绩效

The QIAamp DSP Circulating NA Kit易于使用，并符合GMP要求制造。此试剂盒可分离和纯化高品质核酸用于下游应用。在QIAvac 24 Plus上的真空处理使起始样本体积可达5 ml。灵活的洗脱体积在20 µl到150 µl之间，可对低浓度核酸进行浓缩。

原理

QIAamp DSP Circulating NA Kit使用硅胶膜技术分离和纯化游离的、不含细胞的病毒核酸。

程序

QIAamp DSP Circulating NA Kit的操作流程包括4个步骤（裂解、结合、洗涤和洗脱），在真空底座上使用QIAamp Mini离心柱进行操作。核酸可被有效纯化和浓缩。完成裂解和上样步骤后，可在QIAcube全自动核酸纯化仪上自动纯化游离核酸。

应用

QIAamp DSP Circulating NA Kit极大简化了从以下样本中分离游离的DNA和RNA：

血浆
血清
尿液

辅助数据和图表

QIAamp DSP Circulating NA Kit procedure.

Tube extenders for processing on the QIAvac 24 Plus.

Comparison of Classic and Breeze workflows.

资源

Publications

Implementing prenatal diagnosis based on cell-free fetal DNA: accurate identification of factors affecting fetal DNA yield.

Barrett AN; Zimmermann BG; Wang D; Holloway A; Chitty LS;

PLoS One; 2011; 6 (10):e25202 2011 Oct 4 PMID:21998643

FAQ

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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