Buffer RLT

用于RNA纯化前裂解细胞和组织

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Buffer RLT (220 ml)

Cat. No. / ID: 79216

220 ml RNeasy Lysis Buffer

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产品详情

Buffer RLT裂解液用于在RNA纯化前裂解细胞和组织，适用于RNeasy Plus或Allprep DNA/RNA实验。

资源

Download Safety Data Sheets for QIAGEN product components.

Publications

Transcriptional regulation of uterine vascular endothelial growth factor during early gestation in a carnivore model, Mustela vison.

Lopes FL; Desmarais J; Ledoux S; Gévry NY; Lefevre P; Murphy BD;

J Biol Chem; 2006; 281 (34):24602-11 2006 Jun 21 PMID:16790435

FAQ

The exact composition of Buffer RLT is confidential. This buffer is a proprietary component of RNeasy Kits. Buffer RLT contains a high concentration of guanidine isothiocycanate, which supports the binding of RNA to the silica membrane. Buffer RLT can be purchased separately (cat. no. 79216)

Note: note that ß-mercaptoethanol should be added to Buffer RLT before use to effectively inactivate RNAses in the lysate (10 µl ß-Mercaptoethanol per 1 ml Buffer RLT).

FAQ ID -2793

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12