MagAttract Viral RNA M48 Kit

从多达300 µl的血清或血浆样本中自动纯化病毒RNA

Products

MagAttract Viral RNA M48 Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

特点

  • 快速纯化高品质的病毒RNA
  • 每批可灵活处理6–48个样本
  • 易于优化RNA的浓度

产品详情

MagAttract Viral RNA M48 Kit应用经验证的MagAttract磁珠技术,在BioRobot M48工作站上自动纯化病毒RNA。灵活的洗脱体积,易于优化病毒RNA的浓度,适合各种下游应用。在移液吸头中进行病毒RNA的纯化和磁力分离,提高了整个流程的效率。

绩效

MagAttract Viral RNA M48 Kit纯化一个样本获得的病毒RNA的量通常小于1 µg ,因而采用分光光度计很难定量。推荐使用定量扩增的方法确定RNA的产量。

原理

MagAttract Viral RNA M48 Kit用于从血清和血浆中全自动纯化病毒RNA。MagAttract技术配合硅胶膜技术快速、高效的纯化RNA,硅胶膜覆盖在方便使用的磁珠表面。样本放在1.5 ml或2 ml的样品管中,在BioRobot M48工作站上处理。每批可处理6–48个样本,样本来源为至多300 µl的血清或血浆。纯化获得的RNA的量取决于样本类型和病毒滴度。

程序

MagAttract Viral RNA M48流程中,病毒RNA在离液盐中结合到含有磁珠的硅胶膜表面。随后,结合到磁珠上的病毒RNA使用2种不同的洗涤缓冲液有效洗涤。然后,使用不含RNase的洗脱液,在65ºC条件下,将高品质病毒RNA洗脱。

应用

MagAttract Viral RNA M48 Kit和BioRobot M48工作站共同使用,纯化得到的高品质RNA适用于各种下游应用,包括:

  • 病毒流行病学和基因分型
  • 传染性疾病的研究

辅助数据和图表

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time, genotyping, sequencing
Sample amount300 µl
For automated processingBioRobot M48 Workstation
Elution volume50–100 µl
Main sample typeSerum, plasma
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral RNA
ProcessingAutomated
FormatFilter tip (up to 48 per run)
TechnologyMagnetic particles

资源

Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

Where can I download the protocol for the KingFisher Flex?

You will find a link to the protocol under the Resources tab.

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Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?

Please contact QIAGEN Technical Services.

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Who can help in case of issues with the third party instrument?

Please contact the respective QIAGEN Technical Services.

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Is the virus inactivated during the procedure?

This cannot be proven with the methods we have at our disposal.

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I would like to use the kit on another third party instrument. Who should I contact?

Please contact QIAGEN Technical Services.

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Can I use sample volumes other than 300 µl?

Using the same protocol, 200, 300, and 400 µl can be prepared, with the expected loss or gain in sensitivity.

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Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?

Not yet, but these might be added here in the future. Please contact QIAGEN Technical Services.

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