QuickLyse Miniprep Kit

超快速纯化至多15 μg测序级别纯的质粒DNA

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QuickLyse Miniprep Kit (250)

Cat. No. / ID: 27406

250 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)

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QuickLyse Miniprep Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

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特点

快速的一步裂解
减少手动操作时间和实验步骤
1.5–3 ml菌液至多可获得15 μg DNA
9分钟可获得即用型质粒DNA

产品详情

QuickLyse Miniprep Kit为提取测序级别的质粒DNA提供了一个以快速离心柱为基础的方法。在单一的缓冲液中，细菌细胞重悬、溶解，同时与DNA结合，使得在较短的操作时间内快速纯化DNA。

绩效

QIAGEN QuickLyse Miniprep Kit采用一步裂解技术，可以超速制备至多15 µg质粒DNA。DNA的质量确保了其在下游应用中的良好表现，如测序、限制性分析和克隆（参见" Reproducible yields"）。

QuickLyse Miniprep Kit纯化的足量质粒DNA，可以用于任何的下游应用，包括测序（参见 " Long read-lengths in automated sequencing"）。从1.5 ml的细菌培养液中至多获得15 µg的高拷贝质粒DNA（参见 " High yields of high-quality plasmid DNA"）。纯化得到的质粒DNA有1.7–1.9的OD₂₆₀_/280 比率，表明其质量和纯度适合多种敏感应用。而且很容易获得超过700个单位的读取长度。

查看图表

Reproducible yields.

Long read-lengths in automated sequencing.

High yields of high-quality plasmid DNA.

原理

不同于大多数方案需要3步裂解程序，QuickLyse Miniprep Kit将细菌细胞裂解的酶和渗透压处理步骤融合到一个3分钟步骤中。此外，QuickLyse技术使用很少量的缓冲溶液、简化操作并且节约大量时间：可以在22分钟内获得24份小量纯化的质粒DNA。

程序

重悬浮和裂解的细菌细胞和DNA结合在单一的缓冲溶液中，使得DNA在短时间内得到快速纯化。在3分钟的裂解步骤后，澄清的裂解产物直接加入到QuickLyse Spin Column中。质粒DNA结合在离心柱膜上，然后用简单的清洗步骤洗脱。（参见""）。得到的DNA可以直接运用在标准的应用中，包括自动测序、PCR、限制性分析和克隆。

查看图表

QuickLyse procedure.

应用

QuickLyse Miniprep Kit产生足量的质粒DNA用于多种的下游应用，包括自动测序。

辅助数据和图表

High yields of high-quality plasmid DNA.

Typical DNA yield and purity from 1.5 ml of bacterial culture (LB media) for different cells and vectors.

Specifications

Features	Specifications
Applications	Automated sequencing, PCR, restriction analysis, cloning etc.
Time per run or prep per run	9 min
Culture volume/starting material	1.5 ml culture volume
Plasmid type	High-copy, cosmid DNA
Elution volume	50 µl
Samples per run (throughput)	1–24 samples per run
Technology	QuickLyse technology
Yield	3–7 µg
Processing	Manual (centrifugation)

资源

For purification of sequencing grade plasmid DNA

Download Safety Data Sheets for QIAGEN product components.

FAQ

The QuickLyse Miniprep Kit can be used for plasmids up to 9 kb in size. It is not recommended for Large-Construct purifications.

Please find an overview of additional kit options for small-scale Plasmid Purification on the QIAGEN website, or use our ProductFinder to select the right kit for your needs.

FAQ ID -1752

No, this is not possible since the columns do not fit properly on the QIAVac 24 Plus manifold and the vacuum is not strong enough.

Normally, 1.5 ml bacterial culture is used with the QuickLyse Miniprep Kit. Only if the OD600 is < 2.0, up to 3 ml culture may be used. Growth in LB medium is strongly recommended. Growth in nutrient-rich media, such as 2xYT, is not recommended.

FAQ ID -1357

When using the QuickLyse Miniprep Kit, the addition of 125 µl isopropanol to the lysate, prior to transferring it to the QuickLyse Spin Column, may increase plasmid DNA yield in some cases.

FAQ ID -1354

No. Vortexing for 30 seconds as described in the QuickLyse Miniprep Handbook is absolutely critical to resuspend and lyse the bacterial cells. Failure to follow this step will result in greatly reduced DNA yields.

FAQ ID -1352

Elution Buffer QLE of the QuickLyse Miniprep Kit contains 10 mM Tris-Cl and 0.1 mM EDTA (pH 8.5). Due to the very low concentration of EDTA, enzymatic downstream reactions such as PCR and cycle sequencing are not inhibited.

FAQ ID -1356

Yes, nuclease-free water may be used for the elution of plasmid DNA purified with the QuickLyse Miniprep Kit. However, for higher DNA stability and optimal yields, it is recommend to elute using the provided elution buffer (Buffer QLE).

FAQ ID -1355