QIAprep 96 Plus Kits

96孔板式纯化至多50 μg高品质质粒DNA

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QIAprep 96 Plus Miniprep Kit (4)

Cat. No. / ID: 27291

For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well blocks.

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ForFor instrument

QIAvac 96

BioRobot Universal

QIAprep 96 Plus Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

持续获得至多50 μg的高产量
纯化得到的产品适用于多种应用
真空、离心和自动化多种处理方式可供选择

产品详情

QIAprep 96 Plus Kits以96孔板式制备质粒DNA。使用QIAvac 96和/或离心机处理样本，或在BioRobot Universal System上自动处理。从5 ml E.coli 培养液中每孔可制备至多50 μg高品质质粒DNA（内毒素低于1 EU/μg DNA）。

绩效

高纯度和高产量

QIAprep 96 Plus Kits利用创新的方法制备高纯度DNA（参见"Superior plasmid DNA quality"）。优化的操作流程确保能够持续获得多至50 μg的DNA。该试剂盒内含有独特的结合缓冲液，确保了特定的结合条件（参见"High yields of plasmid DNA" 和 "Superior performance compared to other suppliers"）。

原理

独特的试剂化学和Plasmid Plus 96板设计确保其高效的结合能力。中和后，通过TurboFilter 96板澄清裂解液。澄清后的裂解液中加入非离液结合缓冲液，使得质粒DNA充分结合到Plasmid Plus 96 板的膜上。独特的结合缓冲液确保特定的结合条件，得到的DNA品质相当于使用阴离子交换制备的DNA。独特的试剂盒化学可从5 ml E. coli 培养液中每板纯化多至50 μg质粒DNA。

程序

QIAprep 96 Plus 利用96孔板式制备质粒。QIAprep 96 Plus Miniprep操作方法基于优化的碱裂解法。澄清裂解液后，只需简单的结合–洗涤–洗脱过程即可得到高浓度的质粒DNA，可直接用于下游应用。

方便、灵活处理

QIAprep 96 Plus Miniprep Kit使用QIAvac 96和/或离心机简便处理，也可在BioRobot Universal System上通过QIAprep 96 Plus BioRobot Kit全自动处理。优化的操作方法确保通过96孔板简便的进行质粒DNA制备。

应用

QIAprep 96 Plus Kits纯化的质粒DNA高度适用于多种应用，包括：

制备发夹载体（sh载体）
酶修饰
克隆
限制性酶切
测序

辅助数据和图表

High yields of plasmid DNA.

E. coli DH5 a strains carrying pCMVβ were grown in 5 ml LB medium. Plasmid DNA was isolated manually using the QIAvac 96 and either the QIAprep 96 Plus Miniprep Kit or the QIAprep 96 Turbo Miniprep Kit or automated on the BioRobot Universal System using either the QIAprep 96 Plus BioRobot Kit or the QIAprep 96 Turbo BioRobot Kit. DNA was quantified using absorbance measurement and verified by agarose gel analysis. Higher yields were achieved with QIAprep 96 Plus Miniprep and BioRobot Kits.

Superior performance compared to other suppliers.

Specifications

Features	Specifications
Applications	Fluorescent and radioactive sequencing, cloning etc.
Technology	Silica technology
Time per run or prep per run	<70 minutes
Yield	<20 µg
Culture volume/starting material	1–10 ml culture volume
Samples per run (throughput)	48–96 samples per run
Plasmid type	High-copy, low-copy, cosmid DNA
Processing	Automated

资源

Download Safety Data Sheets for QIAGEN product components.

FAQ

For the manual procedure, the plates can be partially used. Any unused wells should be sealed with tape. For the BioRobot Universal procedure, the entire 96 sample plate must be used. However, different sized plates may be available from our applications lab. Please contact QIAGEN Technical Service for more details.

https://www.qiagen.com/contact/contacttechnicalservice.aspx?WT.svl=m

FAQ ID -2319

For sensitive cell lines and downstream assays, we recommend QIAGEN Plasmid Plus 96 Miniprep Kit, because the endotoxin value is below 1 EU/µg DNA. The QIAGEN Plasmid Plus 96 MiniPrep kit contains a special protocol for endotoxin removal. Combination of other chemistries (such as Buffer ER) is not possible with this kit. For robust cell lines, the QIAprep 96 Plus Miniprep Kit may be okay, as the endotoxin values are below 10 EU/µg DNA.

FAQ ID -2320

The QIAprep 96 Plus Miniprep Kit and the QIAGEN Plasmid Plus 96 Miniprep Kit can be used for the purification of plasmids up to 40 kb.

FAQ ID -2316

The QIAprep 96 Plus Miniprep Kit and the QIAGEN Plasmid Plus 96 Miniprep Kit can be used for the purification of low-copy plasmids.

FAQ ID -2317

Yes, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit are suited for the plasmid purification of short hairpin vectors?

FAQ ID -2318