QIAamp MinElute Media Kit

用于从液体培养基中纯化 DNA

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QIAamp MinElute Media Kit

Cat. No. / ID: 57414

用于 50 次小提：50 个 QIAamp MinElute 离心柱、QIAGEN Proteinase K、载体 RNA、缓冲液、Extension Tubes (3 ml)、Collection Tubes (1.5 ml)

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QIAamp MinElute Media Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

从各种液体运送培养基中纯化
省时的真空程序，方便操作，易于使用
洗脱体积灵活，从 20 至 150 µl 不等
高效去除酒精/污染物的高品质 DNA

产品详情

QIAamp MinElute Media Kit 提供了从宫颈拭子运送培养基等液体培养基中纯化核酸的便捷真空程序。可在 QIAvac 24 Plus vacuum manifold 上快速处理 QIAamp MinElute 柱。使用 QIAamp MinElute Media Kit 进行的 DNA 纯化可在 QIAcube Connect 上自动完成。

绩效

QIAamp MinElute Media Kit 可在 <90 分钟的时间内处理 250 µl 的液体运送和储存培养基样本。在 QIAvac 24 或 QIAvac 24 Plus vacuum manifold 上处理 QIAamp MinElute 柱时，以 24 个样本为一批进行处理。DNA 的洗脱量为 20-150 µl。

原理

QIAamp MinElute Media Kit 采用成熟的核酸纯化技术。该试剂盒将硅胶膜的选择性附着属性与 20-150 µl 的灵活洗脱容量相结合。该试剂盒适用于含有核酸的液体培养基，如宫颈拭子运送培养基（如 PreservCyt 或 SurePath 溶液）。核酸在 Buffer AVE 中洗脱，可随时在扩增反应中使用或存放。纯化的核酸不含蛋白质、核酸酶和其他杂质。

程序

QIAamp MinElute Media 程序包括 4 个步骤（裂解、结合、洗涤、洗脱），是在 QIAvac 24 Plus vacuum manifold 上使用 QIAamp MinElute 柱进行的。该程序设计用于确保消除可检测的样本间交叉污染，以及安全处理具有潜在感染性的样本。简单的 QIAamp MinElute 程序非常适合同时处理多个样本，可在 90 分钟内从 24 个样本中获得纯核酸。

应用

QIAamp MinElute Media Kit 可用于从液体培养基（如宫颈拭子运送培养基）中分离 DNA。该试剂盒可用于从各种来源纯化细胞、细菌和病毒核酸，包括：

含酒精的液体细胞学培养基（如 PreservCyt 和 SurePath）
磷酸盐缓冲液体运送培养基（例如 M4RT）

Specifications

Features	Specifications
Applications	PCR、real-time PCR
Format	MinElute 柱
Time per run or per prep	<90 分钟（24 个样本）
Sample amount	250 µl
Elution volume	20-150 µl
Technology	硅胶膜技术
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	病毒 DNA 和 RNA、细菌 DNA 和 RNA、细胞 DNA 和 RNA
Yield	不一
Main sample type	液体培养基
Processing	手动（真空）

资源

Download Safety Data Sheets for QIAGEN product components.

FAQ

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728