Puregene Accessories

从各种类型的样本中纯化可存档的DNA

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Proteinase K (5 ml)

Cat. No. / ID: 158146

5 ml Puregene Proteinase K

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酶解决方案

Enzyme

Solution

类型

Proteinase K

RNase A

体积

5 ml

650 µl

The implementation of an in-house manufacturing solution has lead to product name and catalogue number changes in the Puregene portfolio . This change will not reflect on the quality and performance of the products.

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

高品质DNA用于长期储存
高重复性的DNA纯化
方便、可扩展的纯化操作

产品详情

Puregene Accessories配合Gentra Puregene Kits纯化高分子量（100–200 kb）DNA，适合用于存档。可扩展的纯化操作，能温和的去除大体积样本中的污染物和抑制剂，纯化获得的大体积样本适合长期保存。

绩效

Catalog number changes in the Puregene line.
NEW product name	NEW cat.no.	Old product name	Old cat. no.
158103	Puregene RBC Lysis Solution (450 ml)	158902	RBC Lysis Solution(450 ml)
158106	Puregene RBC Lysis Solution (1000 ml)	158904	RBC Lysis Solution (1000 ml)
158113	Puregene Cell Lysis Solution (125 ml)	158906	Cell Lysis Solution (125 ml)
158116	Puregene Cell Lysis Solution (1000 ml)	158908	Cell Lysis Solution (1000 ml)
158123	Puregene Protein Precipitation Sol. (50 ml)	158910	Protein Precipitation Solution (50 ml)
158126	Puregene Protein Precipitation Solution (350 ml)	158912	Protein Precipitation Solution (350 ml)
158133	Puregene DNA Hydration Solution (100 ml)	158914	DNA Hydration Solution (100 ml)
158136	Puregene DNA Hydration Solution (500 ml)	158916	DNA Hydration Solution (500 ml)
158143	Proteinase K (650 µl)	158918	Puregene Proteinase K (650 µl)
158146	Proteinase K (5 ml)	158920	Puregene Proteinase K (5 ml)
158153	RNase A Solution (650 µl)	158922	RNase A Solution (650 µl)
158156	RNase A Solution (5 ml)	158924	RNase A Solution (5 ml)
158183	Glycogen Solution (500 µl)	158930	Glycogen Solution (500 µl)
158186	Glycogen Solution (5 ml)	949002	Autopure Glycogen Solution (5 ml)

原理

应用Gentra Puregene Kits，细胞用阴离子洗涤剂裂解，其中加入DNA稳定剂。DNA稳定剂限制胞内及其他环境中的DNases的活性。使用RNA降解酶去除RNA。蛋白质等其他污染物经盐析沉淀法去除。最终，通过乙醇沉淀回收基因组DNA，水合溶液（1 mM EDTA、10 mM Tris·Cl pH 7.5）溶解DNA。一般情况下，纯化的DNA的A₂₆₀/A₂₈₀值在1.7–1.9之间，大小可达200 kb。纯化的DNA可贮存于2–8°C、–20°C或–80°C，确保安全。

程序

可存档的DNA

DNA的纯度对下游应用结果的精确性至关重要，诸如PCR等敏感的下游应用要求使用最高品质、且分子量尽可能大的DNA，以确保可靠的结果。Gentra Puregene Kits去除污染物和酶抑制剂，确保获得高度稳定的适合存档的DNA。纯化获得高品质的DNA，分子大小为100–200 kb。

应用

应用Puregene Accessories和Gentra Puregene Kits纯化的DNA高度稳定，适用于各种下游应用，包括：

DNA存档
PCR
SNP分析
Southern印迹

资源

Publications

Methylation variation at IGF2 differentially methylated regions and maternal folic acid use before and during pregnancy.

Hoyo C; Murtha AP; Schildkraut JM; Jirtle RL; Demark-Wahnefried W; Forman MR; Iversen ES; Kurtzberg J; Overcash F; Huang Z; Murphy SK;

Epigenetics; 2011; 6 (7):928-36 2011 Jul 1 PMID:21636975

FAQ

The exact composition of RBC Lysis Solution is confidential. RBC Lysis Solution is a component of Gentra Puregene Blood Kits. RBC Lysis Solution selectively lyses human red blood cells leaving white blood cells intact. You can order RBC Lysis Solutin separately.

FAQ ID -2808

The composition of DNA Hydration Solution is as follows:

10 mM Tris
1 mM EDTA
pH 7–8

DNA Hydration Solution is included in QIAGEN’s Gentra Puregene Kits for DNA purification and can also be bought separately from QIAGEN.

FAQ ID -2813

The detailed composition of Cell Suspension Solution is confidential. Cell Suspension Solution is a component of the Gentra Puregene Yeast/Bacteria Kit. Essentially, it is an isotonic solution that prevents spheroblasts from bursting. Cell Suspension Solution can be ordered separately.

FAQ ID -2811

The exact composition of Protein Precipitation Solution is confidential. Protein Precipitation Solution is a component of Gentra Puregene Kits for DNA purification. Essentially, this is a high-salt buffer that lowers the solubility of proteins. Protein Precipitation Solution can be ordered separately.

FAQ ID -2810

The exact composition of Glycogen Solution is confidential. The Glycogen concentration is 20mg/mL. Glycogen Solution is a component of Gentra Puregene Kits for DNA purification. During isopropanol precipitation, Glycogen Solution acts as a nucleic acid carrier and helps to efficiently precipitate small amounts of DNA. In addition, it facilitates visualization of the DNA pellet. Glycogen Solution can be purchased separately.

Note: Glycogen Solution does not work in combination with QIAGEN’s silica membrane technologies, e.g., RNeasy, DNeasy, QIAamp, etc. With these technologies, poly-A carrier RNA is used when dealing with low amounts of nucleic acids.

FAQ ID -2812

The exact composition of Cell Lysis Solution is confidential. Cell Lysis Solution is a component of Gentra Puregene Kits for DNA purification. It functions by enabling cell lysis. Cell Lysis Solution can be purchased separately.

FAQ ID -2809

The Puregene RNase A and Autopure RNase A solutions both have a concentration of 4 mg/ml.

FAQ ID -3083

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618