连接计算器
此工具确定了在适用于典型连接反应的范围内的各种插入片段:载体摩尔比率所需的插入片段质量。
见解
所需的 DNA 插入片段质量 [g] = 载体 DNA 质量 [g] x {DNA 插入片段长度 / 载体 DNA 长度} x 期望的插入片段与载体摩尔比率
提示：期望的比率为集合 {1, 2, 3, 5 ,7} 中的一个
插入片段长度应小于载体长度
阅读更多
推荐产品
CAT NO./ID 203123
AllTaq PCR Core Kit (250 U)
50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
CAT NO./ID 201203
Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
CAT NO./ID 19101
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution)
Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
CAT NO./ID 203203
HotStarTaq DNA Polymerase (250 U)
250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2
CAT NO./ID 205111
Omniscript RT Kit (50)
For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water
CAT NO./ID 79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps