QIAsymphony PAXgene Blood RNA Kit

从稳定的血液中自动纯化胞内RNA（包括miRNA）

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QIAsymphony PAXgene Blood RNA Kit (96)

Cat. No. / ID: 762635

For 96 preps: 2 Reagent Cartridges, Enzyme Racks, accessories, and RNase-free buffers

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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

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特点

提高RNA品质和产量
有效的miRNA共纯化
操作安全、易于使用的创新预装试剂条
标准化的处理流程实现高度重复性
条形码扫描追踪样本和试剂

产品详情

QIAsymphony PAXgene Blood RNA Kit提供全自动的胞内RNA 纯化，适用于QIAsymphony SP核酸提取纯化分析仪。创新的改进试剂盒确保了更高品质的产物RNA，包括miRNA。与PAXgene Blood RNA Tubes（由BD和BD授权代理商提供，货号762165）配合使用进行样本采集和稳定，此系统采用经验证的磁珠技术，提供一个完整的分析前处理方案，用于样本采集和稳定及纯化高品质的RNA。

绩效

全自动的PAXgene Blood RNA实验方案可获得与手动PAXgene Blood RNA实验方案相当的 RNA产量，通常2.5 ml健康人类全血（4.6 x 10⁶ – 1.1 x 10⁷白细胞/ml）的RNA产量为≥3 μg，其中>95%的样品经PAXgene Blood RNA Kits处理。自动化的PAXgene Blood RNA实验方案可从不同供体样本中纯化高品质小分子RNA（参见" High-quality RNA from different donors"）。由于产量与供体高度相关，个体产量可能不同。DNase处理可降低基因组DNA污染。纯化得到的RNA可以即用于下游应用，如real-time RT-PCR。

查看图表

High-quality RNA from different donors.

原理

QIAsymphony PAXgene Blood RNA Kit提供全自动的胞内RNA 纯化，适用于QIAsymphony SP工作站。与PAXgene Blood RNA Tubes（由BD和BD授权代理商提供，货号 762165）配合使用进行样本采集和稳定，此系统采用经验证的磁珠技术，提供一个完整的分析前处理方案，用于样本采集和稳定及纯化高品质的 RNA。预装试剂条将手工操作减少到最小，通过条形码可实现试剂全程追踪。

程序

将血液样品（2.5 ml）收集在PAXgene Blood RNA Tubes中，可在室温下储存或运输。纯化过程的第一步是通过离心使每个PAXgene Blood RNA Tube中的样品沉淀，沉淀重悬后，将试管转移到QIAsymphony SP工作台上。载入的试剂盒和耗材由专用软件指导和检查。之后QIAsymphony SP工作站就可开始全自动的RNA纯化过程。DNase处理确保将基因组DNA污染最小化（参见""）。灵敏和标准化的操作流程配合QIAsymphony SP工作站内部处理，可将样品与样品之间的交叉污染最小化（参见""）。纯化之后，通过最后加热处理，提高洗脱液在下游应用中的表现。处理24个样品的整个流程大约2.5小时，其中手工操作时间最多为25–30分钟。

查看图表

QIAsymphony PAXgene Blood RNA procedure.

On-board measures against cross-contamination.

应用

使用QIAsymphony PAXgene Blood RNA Kit和QIAsymphony SP核酸提取纯化分析仪可纯化得到高纯度RNA，可广泛地用于各种敏感的下游应用，包括：

RT-PCR和定量real-time RT-PCR
表达谱芯片分析
cDNA合成
RNase和S1核酸酶保护分析
Northern印迹、点印迹和狭缝印迹

辅助数据和图表

High-quality RNA from different donors.

Blood was collected from 12 donors into PAXgene Blood RNA Tubes. RNA was purified using the QIAsymphony PAXgene Blood RNA Kit on the QIAsymphony SP. RNA was analyzed with an Agilent 2100 Bioanalyzer. Clear enrichment of small RNA species is shown in the gel-like image by the corresponding peaks in the donor RNA next to the marker band.

Specifications

Features	Specifications
Applications	PCR, qPCR, real-time PCR
Time per run	Varies
Processing	Manual (centrifugation or vacuum)
Format	96-well plate
Sample amount	25–100 mg
Yield	Varies
Main sample type	Tissue samples
Elution volume	45–70 µl
Technology	Silica technology

资源

For collection, transport, and storage of whole blood and stabilization and purification of intracellular RNA

For collection of stable blood samples and purification of high-quality RNA for demanding downstream applications

Download Safety Data Sheets for QIAGEN product components.

For use with software version 5.0 or higher

For purification of total RNA, including miRNA, from human whole blood stabilized in PAXgene Blood RNA Tubes using the QIAsymphony SP and QIAsymphony PAXgene Blood RNA Kit.

Typical total RNA yields from PAXgene Blood RNA Tubes processed with the QIAsymphony PAXgene Blood RNA Kit

Voss et al., AMP 2010

FAQ

Room temperature storage of the filled PAXgene Blood RNA Tubes for up to three days has no effect on the RNA profile. For longer storage they should be stored at –20°C or –70°C.

FAQ ID -2984

RNA yields isolated from 2.5 ml healthy, human, whole blood (4.8 x 10⁶ –1.1 x 10⁷ leukocytes/ml) are specified to ≥3 µg for >95% of the samples processed (see Technical Note for the QIAsymphony PAXgene Blood RNA System ). Since yields are highly donor-dependent, individual yields may vary. Mean yields in the range of 8-10µg per tube or higher.

FAQ ID - 3475

Stability data are not yet available. A study is ongoing.

FAQ ID -2983

Denaturation of the eluate is essential for maximum efficiency in downstream applications, such as RT-PCR, other amplification reactions, or cDNA synthesis. It is not necessary to denature samples more than once; samples remain denatured after freezing and thawing.

FAQ ID -2987

We recommend using Elution Microtubes CL, racked (provided) for elution. Alternatively, 2 ml Sarstedt tubes can be used. Other elution formats will be supported in the future, so please refer to the current labware list.

FAQ ID -2986

The product is not for the isolation of genomic DNA or viral nucleic acids from human whole blood.

FAQ ID - 3476

Due to limited plastic consumables on board the instrument, the number of batches for the QIAsymphony PAXgene Blood RNA protocol is limited to three at one time. Therefore up to 72 samples can be processed in a single run.

FAQ ID -2498

Yes, samples with the QIAsymphony PAXgene Blood RNA Protocol have to be incubated at least 2 hours at room temperature (18– 22°C). Incubation of the PAXgene Blood RNA Tube overnight may increase yields slightly in some cases. Room temperature storage for up to 3 days may have no effect on the RNA profile. During the preparation of the RNA the manual pretreatment consists of a centrifugation and a pellet resuspension step (see the QIAsymphony PAXgene Blood RNA Kit Handbook).

FAQ ID -2982

No. The QIAsymphony PAXgene Blood RNA System is ’For Research Use Only’. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. The performance characteristics of this product have not been fully established.

FAQ ID -2496

The QIAsymphony PAXgene Blood RNA Protocol uses 2.5 ml whole human blood collected in PAXgene Blood RNA Tubes.

FAQ ID -2981

The QIAsymphony PAXgene Blood RNA Kit can be used for 96 RNA isolations.

FAQ ID -2497