QIAamp DSP Virus Kit

从人类血清或血浆中纯化病毒核酸

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QIAamp DSP Virus Kit

Cat. No. / ID: 60704

For 50 preps: QIAamp MinElute Columns, buffers, reagents, tubes, column extenders, VacConnectors

Inquire

QIAamp DSP Virus Kit 旨在用于体外诊断用途。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

通用的核酸纯化系统
获得高品质病毒核酸
浓缩的核酸，洗脱体积为20 μl或60 μl
上样量为500 µl
快速纯化，将交叉污染的风险降至最低

产品详情

QIAamp DSP Virus Kit采用成熟和方便的QIAamp技术同时纯化病毒DNA和RNA。

绩效

使用QIAamp DSP Virus Kit纯化的病毒核酸可即用于各种敏感的下游应用，如基于酶的扩增或其他修饰，包括：PCR和RT-PCR。

原理

QIAamp DSP Virus Kit采用成熟和方便的QIAamp技术同时纯化病毒DNA和RNA。QIAamp硅胶膜特异性结合裂解样品中的核酸，其他裂解物通过真空操作去除。经过有效地洗涤去除污染物，然后洗脱DNA，洗脱体积为20 µl或60 µl。

程序

QIAamp DSP Virus Kit操作流程包括以下步骤：裂解、结合、洗涤（3x）、离心干燥和洗脱。使用真空抽滤装置，如QIAvac 24 Plus，从血浆或血清中纯化病毒核酸（参见"Procedure"）。

应用

从血浆或血清样本中纯化病毒核酸。样本可加抗凝剂柠檬酸盐或EDTA，可以是新鲜的、或冷冻的（不可重复冻融）。

QIAamp DSP Virus Kit可纯化各种病毒核酸，与各种上游样品收集系统和下游应用兼容。

辅助数据和图表

QIAamp DSP Virus Kit procedure.

Universal nucleic acid purification system that easily fits into diagnostic workflows.

Specifications

Features	Specifications
Applications	PCR, RT-PCR, LCR
Elution volume	20 µl, 60 µl
Main sample type	Serum, plasma
CE/FDA/IVD compatible	CE/IVD
Format	Sample tubes
Processing	Manual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Viral DNA, viral RNA
Sample amount	500 µl
Technology	Silica technology
Yield	Varies

资源

Download Safety Data Sheets for QIAGEN product components.

Publications

Occurrence of hepatitis A virus genotype III in Germany requires the adaptation of commercially available diagnostic test systems.

Heitmann A; Laue T; Schottstedt V; Dotzauer A; Pichl L;

Transfusion; 2005; 45 (7):1097-105 2005 Jul PMID:15987353

FAQ

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

FAQ ID -528

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12