AllPrep RNA/Protein Kit

从细胞中同时纯化RNA和蛋白质

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AllPrep RNA/Protein Kit (50)

Cat. No. / ID: 80404

50 AllPrep Mini Spin Columns, 50 RNeasy Mini Spin Columns, 50 Protein Cleanup Mini Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers

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AllPrep RNA/Protein Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

从同一样本中纯化获得高品质RNA和蛋白质
采用离心柱法快速纯化
采用较少的细胞高效的进行样本制备
获得高品质的RNA和天然蛋白

产品详情

AllPrep RNA/Protein Kit可以从同一个细胞培养样本中同时纯化总RNA和天然蛋白。

绩效

AllPrep RNA/Protein Kit适用于从同一个细胞培养样本中同时纯化总RNA和天然蛋白。使用Agilent 2100 Bioanalyzer对纯化的RNA进行分析通常获得的RIN值为10，表明RNA十分完整。纯化的天然蛋白包含所有细胞蛋白（参见""）。AllPrep RNA/Protein Kit纯化得到的RNA适用于real-time RT-PCR（参见""），纯化得到的细胞蛋白质可用于酶分析（参见""）。

查看图表

Highly intact RNA and high-quality protein from the same cell sample.

Sensitivity in real-time RT-PCR.

Purification of functional protein.

原理

AllPrep RNA/Protein Kit适用于从同一个细胞培养样本中同时纯化总RNA和天然蛋白。该试剂盒可进行高效的样本制备，使用较少量的细胞即可进行纯化，因此适用于在mRNA和蛋白质水平评估siRNA引起的基因敲除。此外，因为RNA和蛋白质来自同一样本，所以基因敲除分析更加可靠。AllPrep RNA/Protein Kit是QIAGEN RNAi和基因表达分析产品线的一部分，可获得具有高度可重复性且灵敏的结果。该试剂盒包含优化的PCR预混液、即用型试剂、siRNA和转染试剂。该产品使RNAi和基因表达分析工作流程标准化，在生物医学研究的各个领域取得了成功。

程序

该试剂盒利用离心柱法，可在40分钟内从同一个细胞样本中纯化RNA和蛋白质（参见""）。AllPrep DNA/RNA/Protein Kit可从培养细胞和组织中同时纯化DNA、RNA和蛋白质（适用于Western印迹，不适用于酶分析）。

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AllPrep RNA/Protein procedure.

应用

AllPrep RNA/Protein Kit适用于从同一个细胞培养样本中同时纯化总RNA和天然蛋白。纯化得到的RNA和蛋白质在下游应用中表现出色，如real-time RT-PCR、Western印迹和酶分析。（注：待测蛋白的功能需要根据其特性进行检测）。

辅助数据和图表

Highly intact RNA and high-quality protein from the same cell sample.

Total RNA was purified from HeLa cells and analyzed on the Agilent 2100 Bioanalyzer. The rRNA ratio (28S/18S) was 2.04. Total protein was purified from HeLa cells and analyzed by western blot. Analysis of duplicate protein samples with antibodies against Lamin (numclear protein) and TIM23 (mitochondrial protein) demonstrates that the total protein fraction contains proteins from subcellular compartments.

Specifications

Features	Specifications
Applications	PCR, real-time PCR, blotting, microarray
Elution volume	30–50 µl
Format	Spin column
Processing	Manual (centrifugation)
Technology	Silica technology
Time per run or per prep	40 minutes
Sample amount	700 µl
Main sample type	Cultured cells
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA, native protein

资源

For simultaneous purification of total RNA and protein from the same animal cell sample

Download Safety Data Sheets for QIAGEN product components.

For simultaneous purification of total RNA and protein from the same animal cell sample

FAQ

As this is a proprietary reagent, the exact composition of Buffer APL is confidential. Buffer APL is an important component of the AllPrep RNA/Protein Kit.

FAQ ID -2803

No. The AllPrep RNA/Protein Kit has been optimized for cultured cells. If you would like to recover RNA and protein from tissue samples, please follow our Supplementary Protocol 'Acetone precipitation of protein from Buffer RLT or Buffer RLT Plus lysates', (RY22).

FAQ ID -1095

The maximum number of cells that can be used with the AllPrep RNA/Protein Kit is 6 x 10⁵ cells per preparation. Using larger cell numbers may reduce RNA as well as protein yield and quality.

FAQ ID -1208