EZ1 DSP Virus Kit

应用EZ1 Advanced或EZ1 Advanced XL从1–6个或1–14个人类血浆、血清或脑脊液样本中自动、同步地纯化病毒DNA和RNA

S_1084_5_GEN_V2

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✓ 博学专业的产品和技术支持

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EZ1 DSP Virus Kit (48)

Cat. No. / ID:   62724

For 48 viral nucleic acid preps: Prefilled Reagent Cartridges, Disposable Tip-Holders, Disposable Filter-Tips, Sample Tubes, Elution Tubes, buffers, carrier RNA
EZ1 DSP Virus Kit 旨在用于体外诊断用途。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 更高的分析灵敏度,可用于处理低滴度病毒
  • 每轮可快速、灵活处理1–6个或1–14个样本
  • 程序设置简单,只需插卡即可运行
  • 标准化的流程,确保可重复性的结果

产品详情

EZ1 DSP Virus Kit包含所有需要的试剂和实验耗材,可运用磁性颗粒技术从人体血清、血浆、脑脊髓液(CSF)、尿液、全血、粪便、传输介质、呼吸样本和干拭子中自动纯化病毒核酸和细菌DNA。试剂都以预装试剂盒的形式提供,确保配合EZ1 Advanced或EZ1 Advanced XL使用的速度和便利性。EZ1 DSP Virus Kit具有精确的性能规格,确保高度可靠的病毒核酸纯化。

绩效

High analytical sensitivity with lower detection limits

The EZ1 DSP Virus Kit provides simultaneous purification of viral nucleic acids and bacterial DNA from human serum, plasma, CSF, stool and nasopharyngeal swabs in UTM. Efficient purification of viral RNA and DNA results in sensitive analytical detection in polymerase chain reaction (PCR), on a variety of real-time thermal cyclers.

原理

自动化样本制备确保高效、标准化的核酸纯化。EZ1 Advanced和EZ1 Advanced XL工作站使用经验证的磁珠技术纯化核酸。试剂产品受严格的质量控制,确保可靠、稳定的性能。易用的仪器、预封装的试剂条、简单的实验方案和操作平台的设置有助于减少手动操作的误差,无论是初学者还是熟练者均可运行该体系。尽量减少了手动操作可能具传染性样本,确保使用者的安全和可靠的样品处理过程。纯化的病毒DNA和RNA可即用于基于酶扩增的敏感下游诊断分析,如通过PCR和反转录PCR(RT-PCR)进行病毒量监测。

程序

EZ1 Advanced或EZ1 Advanced XL工作站与经验证的EZ1 DSP Virus Kit配合使用,可从1–6(EZ1 Advanced)或1–14(EZ1 Advanced XL)个样本中纯化病毒核酸和细菌DNA,样本包括人体血清、血浆、CFS、尿液、全血、粪便、传输介质、呼吸样本和干拭子。从穿透试剂条到洗脱高纯度核酸,所有操作流程由工作站执行。该工作站的创新点在于将吸量管作为分离腔、提高磁珠纯化的效率和避免一系列离心操作。优化的操作步骤使手动操作时间最少,实现高度自动化的核酸纯化。

应用

EZ1 DSP Virus Kit配合EZ1 Advanced DSP Virus Card和EZ1 Advanced或者EZ1 Advanced XL DSP Virus Card和EZ1 Advanced XL,提供了从人体血清、血浆、CSF、尿液、全血、粪便、传输介质、呼吸样本和干拭子中同时纯化病毒核酸和细菌DNA的全自动操作流程。使用EZ1 DSP Virus Kit纯化的病毒核酸可即用于基于PCR和RT-PCR等酶扩增反应的下游诊断分析。

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time RT-PCR, genotyping
Elution volume75–50 µl
Main sample typeSerum, plasma
CE/FDA/IVD compatibleCE/IVD
ProcessingAutomated
For automated processingBioRobot EZ1
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
Sample amount100–400 µl
TechnologyMagnetic particles

资源

试剂盒操作手册 (3)
For the Directive 98/79/EC (IVDD) compliant kit (kit version 4)
EZ1 DSP Virus Kit Handbook_V5_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
New 2D barcodes on EZ1 DSP reagent cartridges
性能数据 (2)
EZ1 DSP Virus Kit Performance Characteristics_V5_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (3)
For the Directive 98/79/EC (IVDD) compliant kit (kit version 4)
New 2D barcodes on EZ1 DSP reagent cartridges
Performance Data (2)
EZ1 DSP Virus Kit Performance Characteristics_V5_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

FAQ

What do I have to do if precipitates are visible in the lysis buffer (well 1 of the reagent cartridge [RCB])?
Place the reagent cartridges (RCB) into a shaker–incubator, and incubate at 30–40°C with mild agitation for up to 2 hours. Do not use the reagent cartridges (RCB) if the precipitates do not redissolve.
FAQ ID -1568
Can I use sample preparation cartridges from other suppliers together with the EZ1 instruments or EZ2 Connect MDx?
No. The EZ1 instruments or the EZ2 Connect MDx are intended to be used only with QIAGEN sample preparation cartridges.
FAQ ID -1567
How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?
For short-term storage of up to 24 hours, it is recommended to store the purified viral nucleic acids or bacterial DNA at 2–8°C. For long-term storage of more than 24 hours, it is recommended to store at –80°C for up to 12 months or –20°C for up to 12 weeks. Stability of nucleic acids might be different for the specific downstream application being used and needs to be self-validated by the user.
FAQ ID - 3897
How can I track samples during collection, sample preparation, and downstream assay?
A sample sheet template is included in the handbook. This sample sheet may be useful for recordkeeping when using the EZ1 DSP DNA Blood or the EZ1 DSP Virus procedure. This sheet can be photocopied and labeled with descriptions of the samples and details of the run.
FAQ ID -1570