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QIAsymphony DNA Investigator Kit (192)
Cat. No. / ID: 931436
For 192 preps of 200 µl each from casework and reference samples: includes 2 reagent cartridges, 2 enzyme racks and accessories
The QIAsymphony Investigator Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Purification from forensic and human identity samples
- More efficient yields from trace casework samples
- Higher signal-to-noise ratios
- Novel, prefilled reagent cartridges for ease of use
- High reproducibility through standardized processing
Product Details
The QIAsymphony DNA Investigator Kit enables automated purification of genomic DNA from 1-96 samples, such as swabs, filters, casework or crime-scene samples, and blood on the QIAsymphony SP. Purification is efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios. The QIAsymphony DNA Investigator Kit meets ISO 18385 requirements.
Performance
The QIAsymphony DNA Investigator Kit enables highly efficient purification of DNA, even from very small amounts of sample material. The combination of sample input volumes of up to 1000 µl with elution volumes as low as 30 µl enables highly sensitive detection of DNA from a wide range of sample types, including diffuse stains.
Principle
The QIAsymphony DNA Investigator Kit combines the speed and efficiency of silica-based purification of genomic and mitochondrial DNA with the convenient handling of magnetic particles (see figure " Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the "Reagents and Consumables" drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. For increased flexibility, DNA can be eluted in volumes of between 30 and 400 µl of water or Buffer ATE (modified TE buffer).
See figures
Procedure
The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 µl to 400 µl of water or modified TE Buffer (Buffer ATE), depending on the protocol.
Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 µl, 500 µl or 1000 µl.
To meet the specific challenges of forensic samples, we have developed sample preparation protocols that ensure the highest possible first-pass success rates. Advanced protocols (ADV) use a heated binding step and increased binding time to maximize yields of pure DNA from the most difficult samples. High-Efficiency (HE) protocols address the challenge of recovering sufficient DNA from touch samples and other low-template samples. TopElute Fluid maximizes sensitivity for these difficult samples. The full range of high-efficiency forensic protocols available for different reference and casework samples is described in the table below.
| Protocol | Lysis volume options | Enhanced binding | Eluate volume | TopElute Fluid |
|---|---|---|---|---|
| Reference | 200, 500 µl | No | 100–400 µl | No |
| Casework | 200, 500, 1000 µl | No | 100–200 µl | No |
| Casework ADV | 200, 500, 1000 µl | Yes | 100–200 µl | No |
| Casework HE | 200, 500, 1000 µl | No | 30–80 µl | Yes |
| Casework HE ADV | 200, 500, 1000 µl | Yes | 30–80 µl | Yes |
Applications
The high-quality DNA obtained using the QIAsymphony DNA Investigator Kit is suitable for direct use in forensic, biodefense, biosecurity, and other human identity applications, such as:
- Genotyping, including fingerprinting and paternity analysis
- DNA purification from trace samples (e.g., crime-scene)
- Routine analysis of reference samples
Supporting data and figures
Efficient processing of magnetic particles.
The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.
Specifications
| Features | Specifications |
|---|---|
| Applications | STR analysis, (real-time) PCR |
| Yield | < 3 µg |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA |
| Elution volume | Reference samples: 100-400 µl; Casework samples: 30-200 µl |
| Sample type | Wide range of forensic and human-identity sample materials |
| Input volume | Reference samples: 200 or 500 µl lysate; Casework samples: 200, 500 or 1000 µl lysate |
| Technology | Magnetic particle technology |
Resources
Validation Reports (2)
Additional Resources (3)
Application/Protocol Documents (14)
Brochures & Guides (4)
Safety Data Sheets (1)
Application Notes (2)
Labware Files and Documents (2)
Instrument Technical Documents (4)
Protocol Files (2)
Technical Information (1)
Labware Documents (1)
Scientific Posters (1)
Kit Handbooks (1)
User-Developed Protocols (1)
Certificates of Analysis (1)
Technical Information and Important Notes (1)
FAQ
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?
Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?