QIAsymphony RGQ
Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs
Products
The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN kits indicated for use with the QIAsymphony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application. The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is intended for the applications described in the respective QIAGEN kits handbooks. If the Rotor-Gene Q instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application.
Features
- Unprecedented flexibility
- Workflow optimization and utility
- Maximized ease of use and convenience
- Regulated assays and laboratory-developed tests
- Designed for largest range of applications
Product Details
QIAGEN's premier sample-to-result workflow solution comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and a broad range of ready-to-use assays on the Rotor-Gene Q real-time PCR cycler. QIAGEN continues to expand the QIAsymphony series with new features, assay menus, and workflows.
Explore the virtual demo to learn more about QIAsymphony RGQ.Performance
The QIAsymphony RGQ, in combination with artus QS-RGQ Kits, enables highly sensitive and specific detection of:
- CMV DNA (see figure " Highly sensitive detection of CMV DNA")
- EBV DNA (see figure " Highly sensitive detection of EBV DNA")
- HBV DNA (see figure " Highly sensitive detection of HBV DNA")
- HIV-1 RNA (see figure " Highly sensitive detection of HIV-1 RNA")
- HCV RNA (see figure " Highly sensitive detection of HCV RNA")
See figures
Principle
QIAsymphony RGQ can save you time by processing up to 96 samples, from sample to result, in one integrated run. A dedicated range of kits enable open or closed systems for molecular testing.
QIAsymphony RGQ is the integrated system to maximize your efficiency and optimize your workflows, from sample to result. QIAsymphony RGQ synergizes the automated sample preparation and assay setup on the QIAsymphony SP/AS with manual transfer to the trusted Rotor-Gene Q for detection. The QIAsymphony SP automates sample preparation from a wide range of sample types. The QIAsymphony AS delivers highly reliable and reproducible PCR assay setup and is highly suited for laboratories that perform complex PCR-based molecular testing on a day-to-day basis. The QIAsymphony AS enables setup of multiple assays per run or sample and supports artus QS-RGQ products, for a complete CE-IVD compliant workflow, as well as in-house assays. The Rotor-Gene Q enables sensitive, precise real-time PCR analysis with CE-IVD-marked artus QS-RGQ Kits or in-house assays.
| Kits | Instrument | Method |
|---|---|---|
| QIAsymphony DSP Virus/Pathogen Kits | QIAsymphony SP | Purification of viral nucleic acids or bacterial DNA from a broad range of sample types |
| artus QS-RGQ Kits | QIAsymphony AS | Real-time PCR (multiplex; sample and internal control) |
| Application | Kits | Instrument | Method |
|---|---|---|---|
| Purification | QIAsymphony DSP AXpH DNA Kit | QIAsymphony SP | Purification of DNA from liquid-based cytology media |
| QIAsymphony DSP DNA Kits | QIAsymphony SP | Purification of DNA from human whole blood, buffy coat, tissues, FFPE tissues, cultured cells and bacterial cultures, as well as viral DNA from human whole blood | |
| QIAsymphony DSP Virus/Pathogen Kits | QIAsymphony SP | Purification of viral nucleic acids or bacterial DNA from a broad range of sample types | |
| QIAsymphony Certal Kits | QIAsymphony SP | Purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations | |
| Assay setup — pathogen detection | QuantiFast Pathogen +IC Kits | QIAsymphony AS | Multiplex real-time PCR and RT-PCR using sequence-specific probes |
| QuantiTect Virus Kits | QIAsymphony AS | Highly sensitive detection of multiple viral DNA and RNA targets in the same tube | |
| QIAGEN OneStep RT-PCR Kit | QIAsymphony AS | Sensitive RT-PCR, with simple setup, using any RNA template | |
| Assay setup — gene expression | Rotor-Gene SYBR® Green Kits | QIAsymphony AS | Ultrafast, precise real-time PCR and RT-PCR using SYBR® Green I |
| Rotor-Gene Probe Kits | QIAsymphony AS | Ultrafast, precise real-time PCR and RT-PCR using sequence-specific probes | |
| Rotor-Gene Multiplex Kits | QIAsymphony AS | Ultrafast, precise multiplex real-time PCR and RT-PCR using sequence-specific probes | |
| Assay setup — residual DNA detection | Certal Detection Kits | QIAsymphony AS | Duplex real-time PCR detection of resDNA target and a generic internal control |
Procedure
The QIAsymphony RGQ workflow starts with purification of nucleic acids on the QIAsymphony SP instrument. The eluates, containing purified nucleic acids from the sample preparation procedure, are transferred to the QIAsymphony AS module, which performs assay setup. The assays are then manually transferred to the Rotor-Gene Q for PCR and data analysis.
To suit individual workflow requirements, there are 2 modes of system operation: integrated or independent. In the integrated mode, samples processed on the QIAsymphony SP are transferred automatically to the QIAsymphony AS, reducing manual handling steps and documentation. For added flexibility, the QIAsymphony SP and AS can also be operated independently of each other in independent mode. See " QIAsymphony workflows".
See figures
Applications
The QIAsymphony RGQ is the most versatile system for all molecular testing needs — today and tomorrow. The QIAsymphony RGQ allows consolidation of commerical assays, as well as in-house–developed tests, on a single platform.
The broad range of applications for open and closed systems includes:
- artus transplantation panel (CMV, EBV, HSV, BK virus, and VZV)
- artus blood-borne virus panel (HIV, HCV, and HBV)
- In-house tests
Supporting data and figures
QIAsymphony workflows.
There are 2 modes of system operation: integrated or independent.
Specifications
| Features | Specifications |
|---|---|
| Elution volume | QIAsymphony SP — 30–500 µl (application dependent) |
| Processing | QIAsymphony SP/AS — automated |
| Storage temperature | QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's package Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1) |
| Features | Rotor-Gene Q — dynamic range, 10 orders of magnitude |
| Operating temperature | QIAsymphony SP/AS — 15–32°C (59–89.6ºF) Rotor-Gene Q — 18–30ºC (64–86ºF) |
| Humidity | QIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C Rotor-Gene Q — relative humidity of 10–75% (noncondensing) |
| Pollution level | QIAsymphony SP/AS — 2 Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3) |
| Overvoltage category | II |
| Samples per run (throughput) | QIAsymphony SP — 1–96 samples in batches of 24 Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100 |
| Heat dissipation/thermal load | Rotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour) |
| Protocols/main application on this instrument | QIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials QIAsymphony AS — PCR setup Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM) |
| Technology | QIAsymphony SP — QIAGEN magnetic-particles chemistry Rotor-Gene Q — real-time PCR cycler |
| Input volume | QIAsymphony SP — minimum input volume: 200 µl (application dependent) |
| Optical System | Rotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations |
| Kits or Application Packs designed for this instrument | QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit |
| Place of operation | For indoor use only |
| Power | QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse |
| Software | Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD |
| Thermal performance | Rotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling |
| Typical run time | Rotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent) |
| Weight | QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration) |
| Transportation conditions | QIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's package Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2) |
| Warranty | Rotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs |
| Instrument dimensions | QIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.) |
| Altitude | Up to 2000 m (6500 ft) |
Service Plans
Resources
Application/Protocol Documents (29)
Supporting Software and Tools (1)
Protocol Files (6)
Instrument User Manuals (2)
Instrument Technical Documents (9)
Labware Files and Documents (1)
Brochures & Guides (1)
Application Notes (2)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
The QIAsymphony RNA kit uses RLT plus as lysis buffer. Can samples lysed in RLT and then stored in the freezer be used on the QIAsymphony?
There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?