| Cell Line Species/Tissue: |
Human
/
Cervix |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10% |
Reporter System:
|
CAT, GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
200000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 µg |
Amount of Enhancer (µl):
|
3.2 µl |
Amount of Reagent (µl):
|
10 µl |
Complex Incubation on Cells (hrs):
|
8 hrs |
Analysis Performed Post-Transfection (hrs):
|
48 hrs |
Transfection Efficiency (%):
|
55 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Compared Effectne to Calcium Phosphate.
Transfection efficiency with Calcium Phosphate: 15%
Transfection efficiency with Effectene: 55% |
| References: |
| |