| Cell Line Species/Tissue: |
Human / Myeloid leukemia
/
|
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
12-well |
Number of Cells:
|
2x10e5 |
Percent Confluence(%):
|
20% |
Amount of Nucleic Acid (µg):
|
20µM/3.3µl |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
18 µl |
Complex Incubation on Cells (hrs):
|
48 h, 72 h |
Analysis Performed Post-Transfection (hrs):
|
48 h, 72 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
~85 % |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| Analysis QRT-PCR |
| References: |
| |