| Cell Line Species/Tissue: |
Mouse
/
Muscle Myoblast, Fibroblast |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
15 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
|
Plate Format:
|
100-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
|
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
|
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| C2C12 cells were transiently co-transfected.
Cells were incubated overnight and extracts were prepared 24 h later, normalized by assaying ß-galactosidase activity, immunoprecipitated using anti-CBP antibodies, and analyzed by Western blotting. |
| References: |
| Interaction between Acetylated MyoD and the Bromodomain of CBP and/or p300; Anna Polesskaya et al., Molecular and Cellular Biology, Aug. 2001, p. 5312-5320 |