Transfection Cell Database - Detailed View


Cell Line: Sf9

Cell Line Species/Tissue: spodoptera frugiperda / ovary 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
SF-90011 
Percent Serum (%):
 
Reporter System:
GFP 
Plasmid Purification Method:
QIAfilter Plasmid Kit 
Plate Format:
 
Number of Cells:
 
Percent Confluence(%):
50-60 
Amount of Nucleic Acid (µg):
0.8 
Amount of Enhancer (µl):
6.4 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
48 
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
10% 
 
Any modifications to the protocol?:
 
Notes:
 
References: