Transfection Cell Database - Detailed View


Cell Line: Primary human embryonic lung

Cell Line Species/Tissue: human / lung 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 10% FBS 
Percent Serum (%):
10% 
Reporter System:
b-gal 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
 
Number of Cells:
 
Percent Confluence(%):
70 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
15% 
 
Any modifications to the protocol?:
Followed transfection selection protocol.  
Notes:
 
References: