FAQ-418
What is the composition of buffer P3?
The composition of Buffer P3 is:
- 3.0 M potassium acetate, pH 5.5
Buffer P3 is the neutralization buffer used in QIAGEN's anion-exchange based Kits for plasmid preparation. Details of buffer preparation and storage are presented in Appendix B of the QIAGEN Plasmid Purification Handbook.
Related products
QIAGEN Plasmid Kits
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. See more information regarding principle below.
QIAGEN Large-Construct Kit
The QIAGEN Large-Construct Kit provides gravity-flow, anion-exchange columns for purification of large-molecular-weight DNA. A unique integrated ATP-dependent exonuclease digestion step ensures selective removal of contaminating genomic DNA. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection.
QIAfilter Plasmid Kits
QIAfilter Plasmid Kits provide anion-exchange-based plasmid purification with bacterial lysate clearing by filtration. Purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.
The QIAfilter Plasmid Mega Kit (cat. no. 12281) and the QIAfilter Plasmid Giga Kit (cat. no. 12291) will be discontinued on June 30, 2021. They remain available as long as stocks last.The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. Visit the QIAGEN plasmid kits web page to learn more.
HiSpeed Plasmid Kits
HiSpeed Plasmid Kits provide fast, large-scale, anion-exchange-based plasmid DNA preparation without centrifugation. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.
EndoFree Plasmid Kits
EndoFree Plasmid Kits provide anion-exchange-based endotoxin-free plasmid DNA purification. QIAfilter Cartridges enable fast lysate clearing by filtration. The purified DNA exceeds the purity obtained by 2 x CsCl gradient centrifugation and is suitable for advanced transfection-grade applications. The EndoFree Plasmid Buffer Set can be used for preparations of 10 mega or 5 giga transfection-grade plasmid or cosmid DNA preparations.
The Buffer ER in EndoFree Plasmid Kits has been updated to exclude Triton™ X-114, ensuring REACH compliance and even lower endotoxin levels (<0.04 EU/μg) in line with FDA vaccine standards. The improved buffer reduces incubation time from 30 minutes on ice to just 5 minutes at room temperature.*
*Please note: This update includes adjusted Buffer ER volumes and revised incubation times in the protocol.