The QIAxcel Advanced is a high-resolution capillary electrophoresis system designed to provide a versatile solution to the limitations and bottlenecks of conventional slab-gel electrophoresis. It automates DNA and RNA analyses with a new level of convenience, combining high-performance analysis with increased time and cost efficiency. You can access over 30 application notes demonstrating the wide range of applications of the QIAxcel Advanced System in all areas of life science research using the ApplicationNoteFinder below.
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Quality control of genomic DNA
This application note describes a rapid, reliable and effective method for quality control of purified genomic DNA using the QIAxcel system. The results are highly reproducible and clearly show whether degradation products are present in a sample.
Rapid analysis of deletions/duplications with MLPA®
In this pilot-study, we used the QIAxcel Advanced System for fast and efficient Multiplex Ligation-dependent Probe Amplification (MLPA) analysis. The QIAxcel Advanced System provides a faster and cost-effective alternative to current sequencer-based methods and enables detection of genomic rearrangements with the same resolution and accuracy as using a sequencer.
Identification of 16 respiratory viral targets
The appliction note describes a method using two-tube multiplex reverse-transcription PCR assay followed by amplicon-size separation using the QIAxcel Advanced System for detecting respiratory viruses.
Fish species identification using PCR-RFLP
The QIAxcel Advanced is shown to facilitate the identification of fish species based on PCR-RFLP (restriction fragment length polymorphism) and provides results in less than 8 h without having to handle mutagenic and hazardous products.
Authentication of Basmati rice using SSR-PCR
In this application note, we descibe an SSR-PCR protocol for routine analysis of Basmati rice using the QIAxcel Advanced System, where all Basmati as well as other types of rice can be identified and quantified as DNA percentage of the Basmati variety.
MIRU-VNTR genotyping of M. tuberculosis strains
MIRU-VNTR (mycobacterial interspersed repetitive units – variable number of tandem repeats) genotyping is commonly applied to studies of Mycobacterium tuberculosis strains. The aim of this study was to assess the performance of the QIAxcel Advanced System in M. tuberculosis genotyping and reliability of MIRU-VNTR pattern generation.
Identifying rare mutations in Diamond-Blackfan anemia
Screening a large set of genes for finding rare mutations associated with Diamond-Blackfan anaemia (DBA) required a methodology based on target enrichment technology combined with high-throughput sequencing. To ensure high quality libraries, rapid quality control using the QIAxcel Advanced System was performed at several steps of the library preparation process.
Characterizing immune gene rearrangement profiles in ALL
The application note describes the use of the QIAxcel Advanced for high-throughput IG/TR marker characterization in Acute Lymphoblastic Leukemia (ALL) as a basis for clone-specific molecular disease monitoring.
Detection of genetically modified plants
The QIAxcel system was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.
QIAxcel system — linkage analysis of zebrafish mutants
In this application note, we describe the transfer of methods based on agarose gel electrophoresis for linkage analysis of zebrafish mutants to the QIAxcel system. The simple sequence length polymorphisms (SSLP) marker ‘a‘ was analyzed. Using the QIAxcel system, we were able to resolve size differences of DNA fragments down to a few base pairs, a resolution that was not attainable using conventional agarose gel electrophoresis.
Detection of alternative Tra2-beta regulated splicing
This application note describes how the QIAxcel system was used to successfully determine the splicing pattern of exonic sequences targeted by Tra2β protein isoforms.
Safe and rapid HLA typing
Using the QIAxcel system and data exchange with HLA genotyping software, PCR products from different HLA loci can be analyzed and typed in a few minutes. This method is simple and reliable and significantly reduces hands-on time compared to traditional methods in clinical research.
A. thaliana genotyping with a CAPS marker for a pks3 mutant allele
The QIAxcel system was used for genotyping A. thaliana with CAPS markers, following mutations in the PKS3 gene (At1g18810), and to identify mutants in various crosses.
Reliable detection of B-cell clonality in lymphoproliferations
DNA fragments amplified using multiplex PCR that was developed to detect immunoglobulin and T-cell receptor gene rearrangements were resolved using the QIAxcel system. Accurate size determination enabled identification of the type of B-cell clonality in lymphoproliferation.
Rapid and accurate detection of Y chromosome microdeletions
Y chromosome microdeletions and rearrangements that are relatively common causes of aberrant sperm profiles were detected using the QIAxcel system. Samples were analyzed directly without prior manipulation, and the type of deletion was detected by automatic fragment-size determination.
Marker-assisted selection of wheat lines for udon noodle production
The QIAxcel system was used for analysis of PCR products generated for marker-assisted selection in wheat. The high-throughput capacity of the system allowed large numbers of plants to be quickly and reliably analyzed, making it highly suitable for plant breeding applications.
Rapid and effective genotyping of Cre transgenic mice
Sizes of Cre gene-specific DNA fragments from the Cre gene were unambiguously identified using the QIAxcel system. The accuracy of the system allowed rapid and reliable identification of Cre transgenic mice.
Mapping mutant gene loci in A. thaliana
In this application note, we describe the assessment of simple sequence length polymorphism (SSLP) and cleaved amplified polymorphisms (CAPS) markers in the mapping of mutant gene loci and the homo/hetero examination of known mutant gene loci using the mutated CAPS or derived CAPS (dCAPS) markers. The QIAxcel system enabled high resolution separation of markers.
ERIC-PCR fingerprinting of indigenous S. meliloti strains
The QIAxcel system was successfully used to identify and analyze DNA fingerprints of Sinorhizobium strains. Analysis using the QIAxcel system involved significantly shorter handling and running times compared to conventional methods, providing an effective, reproducible and time-saving method for determining genetic diversity in bacteria.
Clostridium difficile ribotype determination
In this application note, we describe the transfer of methods based on agarose gel electrophoresis for ribotype detection and genotype characterization of Clostridium difficile to the QIAxcel system. Using the QIAxcel system, we were able to detect the fragment pattern characteristic of the 027 ribotype C. difficile strain in both gel and electropherogram views.
Meat species determination using PCR-RFLP
A strategy using PCR followed by restriction fragment length polymorphism analysis (PCR-RFLP) is used to identify meat from various animal species in food. Analysis using the QIAxcel system proved to be less time consuming and allowed electronic documentation as opposed to conventional agarose gel electrophoresis.
Identifying meat species using RFLP-PCR
This study looks at a sensitive method using restriction fragment length polymorphism PCR and the QIAxcel Advanced System for rapid and accurate meat species authentication crucial to the meat industry. Using this native capillary electrophoresis system significantly reduces analysis time and minimizes procedural errors that would influence the accuracy of the analysis.
ISSR-PCR fingerprinting of plant pathogen strains
In this study, ISSR (Inter Simple Sequence Repeat) sequences of individual C. michiganensis subsp. michiganensis strains were amplified using ISSR-PCR and analyzed using the QIAxcel Advanced System. It is a rapid and inexpensive genotyping technique with a wide range of applications, including the characterization of genetic relatedness among the organisms of a population.
Identifying allergenic nut species using the QIAxcel system
This study demonstrates the successful use of the QIAxcel system for the analysis of PCR products as part of an allergen detection workflow. The QIAxcel system enabled a rapid, reliable and inexpensive identification of four nut species.
Mutation screening of c-kit and EGFR prior to Pyrosequencing
The QIAxcel Advanced System was successfully used to separate PCR amplicons and detect mutations in exons of c-kit and EGFR genes found in gastrointestinal stromal tumors (GIST) and non-small cell lung cancers prior to Pyrosequencing. This method using the QIAxcel system can provide high-quality, reproducible determination of deletions and insertions of key genes.
Quality control of total RNA and cRNA for microarray analysis
In this application note, the suitability of the QIAxcel system was assessed for quality control of RNA for microarray analysis. The values obtained for the tested quality control parameters indicate that the QIAxcel system is highly suited for analyzing the quality of total RNA and fragmented or intact cRNA.
High-throughput genotyping of bacteria
The QIAxcel system was successfully used together with the QIAxcel DNA Screening Kit for high-throughput genotyping of bacteria. The QIAxcel system enabled greater sizing accuracy and more sensitive detection than conventional agarose gel electrophoresis.
Analysis of CTG trinucleotide repeats
The QIAxcel system was used to identify the number of CTG trinucleotide repeats present in the myotonin protein kinase (DMPK) gene in a group of individuals with cataracts.
Detecting virulence factors associated with gastroenteritis
The QIAxcel system is highly suitable for PCR-based identification in biomedical research of virulence genes characteristic for diarrheagenic strains of Escherichia coli and Shigella spp.
Microsatellite-instability testing
The QIAxcel system was successfully used for microsatellite-instability testing in biomedical research using 3 mononucleotide markers with normal and tumor tissue from colon.
Detecting Shiga toxin-producing E.coli using multiplex PCR
Using QIAxcel Advanced after a multiplex PCR allowed us to perform efficient and reliable high-throughput screening of cattle feces for the presence of four major E.coli virulence genes and the seven major Shiga toxin-producing serogroups that give rise to infection in humans.
High-throughput processing of VNTR analyses
Fragment sizes automatically calculated using the QIAxcel system were used to determine the number of repeats in variable number tandem repeat (VNTR) loci. Results obtained using the QIAxcel system enabled discrimination of M. tuberculosis strains.
NGS sample quality control
Quality control of targeted enrichment and library preparation steps using the QIAxcel Advanced System is recommended for next-generation sequencing (NGS) applications that use the QIAGEN GeneReader instrument. A protocol describing the evaluation of samples for NGS on the QIAGEN GeneReader platform using the QIAxcel Advanced instrument and ScreenGel Software v1.5 or higher is presented here.
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