QIAsymphony DNA Investigator Kit

For automated purification of DNA from 1–96 samples on the QIAsymphony SP Instrument

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

QIAsymphony DNA Investigator Kit (192)

Cat. No. / ID: 931436

For 192 preps of 200 µl each from casework and reference samples: includes 2 reagent cartridges, 2 enzyme racks and accessories

$1,442.00

The QIAsymphony Investigator Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

Quantity

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Purification from forensic and human identity samples
More efficient yields from trace casework samples
Higher signal-to-noise ratios
Novel, prefilled reagent cartridges for ease of use
High reproducibility through standardized processing

Product Details

The QIAsymphony DNA Investigator Kit enables automated purification of genomic DNA from 1-96 samples, such as swabs, filters, casework or crime-scene samples, and blood on the QIAsymphony SP. Purification is efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios. The QIAsymphony DNA Investigator Kit meets ISO 18385 requirements.

Performance

The QIAsymphony DNA Investigator Kit enables highly efficient purification of DNA, even from very small amounts of sample material. The combination of sample input volumes of up to 1000 µl with elution volumes as low as 30 µl enables highly sensitive detection of DNA from a wide range of sample types, including diffuse stains.

Principle

The QIAsymphony DNA Investigator Kit combines the speed and efficiency of silica-based purification of genomic and mitochondrial DNA with the convenient handling of magnetic particles (see figure ""). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure ""). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the "Reagents and Consumables" drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. For increased flexibility, DNA can be eluted in volumes of between 30 and 400 µl of water or Buffer ATE (modified TE buffer).

See figures

Efficient processing of magnetic particles.

Prefilled, sealed reagent cartridges.

Procedure

The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 µl to 400 µl of water or modified TE Buffer (Buffer ATE), depending on the protocol.

Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 µl, 500 µl or 1000 µl.

To meet the specific challenges of forensic samples, we have developed sample preparation protocols that ensure the highest possible first-pass success rates. Advanced protocols (ADV) use a heated binding step and increased binding time to maximize yields of pure DNA from the most difficult samples. High-Efficiency (HE) protocols address the challenge of recovering sufficient DNA from touch samples and other low-template samples. TopElute Fluid maximizes sensitivity for these difficult samples. The full range of high-efficiency forensic protocols available for different reference and casework samples is described in the table below.

QIAsymphony DNA Investigator protocols
Protocol	Lysis volume options	Enhanced binding	Eluate volume	TopElute Fluid
Reference	200, 500 µl	No	100–400 µl	No
Casework	200, 500, 1000 µl	No	100–200 µl	No
Casework ADV	200, 500, 1000 µl	Yes	100–200 µl	No
Casework HE	200, 500, 1000 µl	No	30–80 µl	Yes
Casework HE ADV	200, 500, 1000 µl	Yes	30–80 µl	Yes

Applications

The high-quality DNA obtained using the QIAsymphony DNA Investigator Kit is suitable for direct use in forensic, biodefense, biosecurity, and other human identity applications, such as:

Genotyping, including fingerprinting and paternity analysis
DNA purification from trace samples (e.g., crime-scene)
Routine analysis of reference samples

Supporting data and figures

Efficient processing of magnetic particles.

The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.