PCR Master Mix Calculator
1. Choose PCR Product you want to work with.
Custom
Volume of template per reaction
µL
Reaction Volume
µL
Number of reactions
Extra reactions
Total volume
µL
Concentration
Volume
stock
Final
per max
for premix
PCR Buffer
x
x
Taq Polymerase
U/uL
U/uL
dNTP-Mix
mM
mM
Primer A
uM
uM
Primer B
uM
uM
Optional: MgCl2
mM
mM
Optional Tracer
x
x
Optional: Q-Solution
x
x
Water
Template DNA
Volume
per max
for premix
PCR Buffer
0 µL
0 µL
Taq Polymerase
0 µL
0 µL
dNTP-Mix
0 µL
0 µL
Primer A
0 µL
0 µL
Primer B
0 µL
0 µL
Optional: MgCl2
0 µL
0 µL
Optional Tracer
0 µL
0 µL
Optional: Q-Solution
0 µL
0 µL
Water
Template DNA
Volume without template
Volume with template