Ligation Calculator

This tool determines the mass of insert needed for various inserts:vector molar ratios within the range suitable for typical ligation reactions.

Enter the data of the targets you are interested in and receive sequencing calculations

Required insert DNA mass [g] = vector DNA mass [g] x {insert DNA length / vector DNA length} x desired insert to vector molar ratio

Notes: Desired ratio is one from set {1, 2, 3, 5 ,7}

Insert length should be smaller than vector length

First, the insert and vector DNA is digested using restriction endonucleases to create DNA fragments with sticky (or blunt) ends. Next, the desired DNA fragments are isolated and subsequently joined followed by treatment with DNA ligase. Finally, the resulting intact vector is transformed into competent E. coli cells, and transformants are identified through suitable genetic selection methods.

Want to know more about the step-by-step process of DNA ligation and its typical reaction? Learn more.

Discover enzymes that you can use for ligating DNAs.

Looking for more enzymes?

Discover high-quality products designed for various molecular biology applications.

Explore enzymes

Related products

CAT NO./ID L6030-HC-L

T4 DNA Ligase (Rapid)

240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer

$749.00

CAT NO./ID L6020L

T7 DNA Ligase (900,000 U)

900,000 U of T7 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer

$474.00

CAT NO./ID P7080L

T4 DNA Polymerase (exo) (2000 U)

2000 U of T4 DNA Polymerase (3000 U/mL) and 10x Blue Buffer. Storage temperature: –25°C to –15°C

$474.00

CAT NO./ID EN11-250

T4 DNA Ligase MBG (2500 U)

5 x 100 μL of T4 DNA Ligase 5U/ μL, 5 x 200 μL 10x T4 Ligation Buffer, 5 x 80 μL of ATP Solution (25mM), 5 x 200 μL of 50% PEG Solution. Storage temperature: Keep at -20°C.

$179.00

CAT NO./ID P7060L

Klenow Fragment (2500 U)

2500 U of Klenow Fragment (0.5 mL at 5000 U/mL) and 10x Blue Buffer (1 x 1.5 mL)

$470.00

CAT NO./ID EN32-050

Saltonase (5000 U)

20 µL x 250 U/µL. Storage temperature: -20°C without a defrost cycle; -80°C for long storage. Stable at -20°C for at least 24 months. No loss in activity was observed after 8 weeks of incubation at 37°C.

$134.00

CAT NO./ID Y9140-LC-L

End-Repair Mix (low concentration)

For 200 low-concentration DNA reactions. End-Repair Mix 1X (0.20 mL), 10X End-Repair Buffer (1 x 1.5 mL) and 1 mM dNTP solution (0.5 mL).

$470.00

CAT NO./ID 203123

AllTaq PCR Core Kit (250 U)

50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water

$219.00

CAT NO./ID 201203

Taq DNA Polymerase (250 U)

250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2

$104.00

CAT NO./ID 19101

RNase A (17,500 U)

2.5 ml (100 mg/ml; 7000 units/ml, solution) Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.

$309.00

CAT NO./ID 203203

HotStarTaq DNA Polymerase (250 U)

250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2

$218.00

CAT NO./ID 205111

Omniscript RT Kit (50)

For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water

$387.00

CAT NO./ID 79254

RNase-Free DNase Set (50)

1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps

$154.00

CAT NO./ID 19155

QIAGEN Protease (7.5 AU)

7.5 Anson units per vial (lyophilized)

$112.00

CAT NO./ID 19131

QIAGEN Proteinase K (2 ml)

2 ml (>600 mAU/ml, solution)

$137.00

How does the QIAGEN Ligation Calculator work?

The QIAGEN Ligation Calculator helps you determine the optimal amounts of DNA fragments and vector needed for a successful ligation reaction. By inputting the sizes and concentrations of your DNA fragments and vector, the calculator provides the precise volumes required for your ligation setup.

Can the ligation calculator be used for different types of DNA ligations?

Yes, the QIAGEN Ligation Calculator can be used for various types of DNA ligations, including sticky end ligations, blunt end ligations, and TA cloning. The calculator is designed to accommodate different ligation scenarios to help optimize your experimental outcomes.

How accurate are the results from the QIAGEN Ligation Calculator?

The results from the QIAGEN Ligation Calculator are based on standard biochemical principles and the input parameters you provide. While the calculator is designed to offer accurate and reliable recommendations, the actual success of your ligation reaction may depend on various factors, including the quality of your DNA and the efficiency of your ligase enzyme.