| Cell Line Species/Tissue: |
Human
/
Lung Cancer cell line |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10 |
Reporter System:
|
Luciferase, GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
300,000 |
Percent Confluence(%):
|
90 % |
Amount of Nucleic Acid (µg):
|
3 µg |
Amount of Enhancer (µl):
|
8 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
6 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
40 |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |