| Cell Line Species/Tissue: |
Human
/
Lung |
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM F12 glutamax + 7.5% FCS |
Percent Serum (%):
|
7.5 |
Reporter System:
|
B-galactosidase |
Plasmid Purification Method:
|
Macherey-Nagel Nucleobond PC500 |
Plate Format:
|
24-well plate |
Number of Cells:
|
20000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
0.75 |
Complex Incubation on Cells (hrs):
|
16 |
Analysis Performed Post-Transfection (hrs):
|
40 |
Transfection Efficiency (%):
|
30 units |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Tried 0.2, 0.4 and 0.6ug DNA with 1.5ul Attractene reagent, and 0.4ug DNA with 0.75, 1.5 and 2.25ul reagent. 0.4ug+0.75ul gave the best results. Trend in the data: the more DNA the better, and the less reagent the better. |
| References: |
| |