Transfection Cell Database - Detailed View


Cell Line: NIH/3T3

Cell Line Species/Tissue: mouse / embryo fibroblast 
Transfection Reagent: Attractene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10% 
Reporter System:
GFP 
Plasmid Purification Method:
 
Plate Format:
6-well 
Number of Cells:
2x 10^5 (seeded one day before transfection) 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
1,2 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
4,5 µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
48 h 
Transfection Efficiency (%):
60% 
 
Any modifications to the protocol?:
 
Notes:
 
References: