| Cell Line Species/Tissue: |
Monkey
/
|
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
3 x 10^5 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1.5 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
10 µl |
Complex Incubation on Cells (hrs):
|
24 hrs |
Analysis Performed Post-Transfection (hrs):
|
48 hrs |
Transfection Efficiency (%):
|
45 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| It was really easy to use because the manual contains protocols for my cell type (COS-7) and detailed information on the number of cells to seed. |
| References: |
| |