| Cell Line Species/Tissue: |
Murine
/
Blood |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
Optimem |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well plate |
Number of Cells:
|
100,000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
5-20 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3-12 µl |
Complex Incubation on Cells (hrs):
|
18 h |
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
50-70 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| The transfections were done in Optimem (reduced serum) medium. |
| References: |
| |