| Cell Line Species/Tissue: |
Mouse
/
monocyte-macrophage |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI 1640 |
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-Well |
Number of Cells:
|
80,000 cells per well |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
50 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3 µl |
Complex Incubation on Cells (hrs):
|
48 hrs |
Analysis Performed Post-Transfection (hrs):
|
48 hrs |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
>95% |
| |
| Any modifications to the protocol?:
|
| 200µl that contain cells and complexes were incubated for 6 hours, then 400µl RPMI+S were added |
| Notes: |
| QIAGEN MAPK1 siRNA, qRT-PCR Analysis; Protocol modification |
| References: |
| |