| Cell Line Species/Tissue: |
Human
/
Colon carcinoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10% FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
12-well plate |
Number of Cells:
|
100.000 cells/well |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1nM; 10nM; 25nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3µl, 6µl, 9µl |
Complex Incubation on Cells (hrs):
|
72h |
Analysis Performed Post-Transfection (hrs):
|
72h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
< 75% |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| Western Blot Analysis |
| References: |
| |