| Cell Line Species/Tissue: |
Human
/
Aortic Endothelial Cells |
| Transfection Reagent: |
RNAiFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
EGM-2 |
Percent Serum (%):
|
2% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
60.000 |
Percent Confluence(%):
|
70% |
Amount of Nucleic Acid (µg):
|
0.5 µg (~100 nM) |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
>90% |
| Knockdown Efficiency (%): |
>90% |
| |
| Any modifications to the protocol?:
|
| complex formation works with Buffer EC-R or medium |
| Notes: |
| |
| References: |
| |