Transfection Cell Database - Detailed View


Cell Line: MEF

Cell Line Species/Tissue: Mouse / Embryonic fibroblasts 
Transfection Reagent: RNAiFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM 
Percent Serum (%):
10% 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
2x10e4 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.5 µg (~100nM) 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
3µl 
Complex Incubation on Cells (hrs):
48h 
Analysis Performed Post-Transfection (hrs):
48h 
Transfection Efficiency (%):
 
Knockdown Efficiency (%):  
 
Any modifications to the protocol?:
No  
Notes:
 
References: