| Cell Line Species/Tissue: |
Human
/
Skin epithelial-like |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
MEM, Pen Strep Gln |
Percent Serum (%):
|
10 % |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
|
Plate Format:
|
6-well |
Number of Cells:
|
5x10e5 |
Percent Confluence(%):
|
80-99 |
Amount of Nucleic Acid (µg):
|
2 µg |
Amount of Enhancer (µl):
|
4 ml |
Amount of Reagent (µl):
|
8 ml |
Complex Incubation on Cells (hrs):
|
3.5 h |
Analysis Performed Post-Transfection (hrs):
|
4.8 h |
Transfection Efficiency (%):
|
52 % |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Tried Effectene and Electroporationin parallel - did not give as good results |
| References: |
| |