| Cell Line Species/Tissue: |
Human
/
Kidney Epithelial |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM w/antibiotics |
Percent Serum (%):
|
10 |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.5 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
5 |
Complex Incubation on Cells (hrs):
|
~16 |
Analysis Performed Post-Transfection (hrs):
|
72-96 |
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| Plated 150,000 cells per well 24 hours before transfection. Dilute 0.5 ug of plasmid DNA into 50 ul of serum free media before adding reagent. |
| Notes: |
| Best conditions found for transfection of 293 cells in 24-well plate. Perfomed proprietary enzyme assay to evaluate results |
| References: |
| |