| Cell Line Species/Tissue: |
Mouse
/
Muscle Myoblast, Fibroblast |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
20 |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
3 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
|
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| After 24 h, the transfected cells were trypsinized and re-plated into growth medium containing 1mg/ml G418 on 12-well plates. |
| References: |
| The Mammalian Target of Rapamycin Regulates C2C12 Myogenesis via a Kinase-independent Mechanism; Ebru Erbay and Jie Chen; The Journal of Biological Chemistry/Vol 276/ No. 39/ Sep 28/ pp.36079-36082, 2001 |