| Cell Line Species/Tissue: |
Human
/
Carcinoma lung epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM 10% FCS |
Percent Serum (%):
|
10 % FCS |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
60-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
60 % |
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
8 µl |
Amount of Reagent (µl):
|
25 µl |
Complex Incubation on Cells (hrs):
|
12 h |
Analysis Performed Post-Transfection (hrs):
|
24 h |
Transfection Efficiency (%):
|
20 % |
| |
| Any modifications to the protocol?:
|
| no |
| Notes: |
| |
| References: |
| |