Transfection Cell Database - Detailed View


Cell Line: HT-1080

Cell Line Species/Tissue: human / epithelioid fibrosarcoma 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
DME 
Percent Serum (%):
10 
Reporter System:
gene of interest 
Plasmid Purification Method:
QIAGEN Plasmid Kits 
Plate Format:
60-mm dish 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
1.25% 
 
Any modifications to the protocol?:
 
Notes:
 
References:
Rasko, R.J., Gottschalk, R.J., Jue, S.F., and Miller A.D. (1997) Improved transfection efficiency of HT-1080, a fibrosarcoma cell line, using SuperFect Reagent. QIAGEN News 1997 No. 2, 8