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Transfection Cell Database - Detailed View


Cell Line: 293

Cell Line Species/Tissue: human / Kidney Epithelial 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
b-gal 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
60-mm dish 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
Amount of Reagent (µl):
25 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
70% 
 
Any modifications to the protocol?:
 
Notes:
The plasmid used in this experiment was 30 kb. Efficiency determined by counting X-gal stained cells. Also tested SuperFect and achieved 30% efficiency.  
References: