| Cell Line Species/Tissue: |
rat
/
hippocampal neurons |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
Neurobasal medium suppl. with B27 |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.5 |
Amount of Enhancer (µl):
|
1.6 |
Amount of Reagent (µl):
|
2 |
Complex Incubation on Cells (hrs):
|
12 |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
1% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Cells propagated from 18 day-old rat embryos. |
| References: |
| |