| Cell Line Species/Tissue: |
human
/
malignant melanoma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
McCoys |
Percent Serum (%):
|
10 |
Reporter System:
|
|
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
T25 Flask |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 |
Amount of Enhancer (µl):
|
8 |
Amount of Reagent (µl):
|
25 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Split cells the day prior to transfection. Cells were placed under selection 24 h post-transfection and then placed under selection. Obtained stables. Effectene yielded 72% efficiency compared to SuperFect. |
| References: |
| |